利用非侵入性多計量影像觀察人體異種皮移植癌細胞之轉植周邊細胞.pptVIP

* * * * * * * * * * Grey dots = values outside this range * * Complete linkage hierarchical clustering of 10 animals for which coregistered VV, PSP and total choline Sufficient to stratify the data into 2 clusters corresponding to each microenvironment, showing the feasibility of computing an in vivo “signature” of metastasis-permissive microenvironments for the PC-3 xenograft model. * pHe maps obtained from the chemical shift of the pHe marker (2-imidazol-1-yl030thoxycarbonyl propionic acid) * HE staining * Hypoxia, visualized by EGFP distribution Quantification of EGFP did not reveal any significant difference either in the intensity of EGFP fluorescence or in the area * Fig A: OnlLungs nodules ya few small clusters of cells were observed in the lungs of mice with s.c. implanted tumors. In contrast, several large nodules were observed in mice with orthotopically implanted tumors Fig B: Metastases were found in 60% livers from orthotopically implanted mice (3/5) but none from s.c. * Hypoxic cancer cells were observed in the lymph nodes of mice with orthotopically implanted tumors Mice with orthotopic tumors showed accumulation of ascites fluid that contained several fluorescing hypoxic cancer cells, s.c. tumors did not result in malignant ascites * * Targeting angiogenesis and vascularization reduced metastasis in preclinical studies * * * 利用非侵入性多計量影像觀察人體異種皮移植癌細胞之轉植周邊細胞環境的許可性 Marie-France Penet,1 Arvind P. Pathak,1 Venu Raman,1 Paloma Ballesteros,2 Dmitri Artemov,1 and Zaver M. Bhujwalla1 Cancer Res 2009; 69: (22). November 15, 2009 Noninvasive Multiparametric Imaging of Metastasis-PermissiveMicroenvironments in a Human Prostate Cancer Xenograft 9512062 司徒嘉穎 9512028 吳倚伶 Introduction Metastasis： Major mortality from prostate cancer Model：Orthotopic , heterotopic Microenvironment(in vivo) Introduction Noninvasive Multiparametric Imaging of Metastasis-PermissiveMicroenvironments in a Human Prostate Cancer Xenograft Introduction Microenvironment(in vivo)