植物乳杆菌CLP0279发酵生产低温SOD培养基优化.PDFVIP

微生物学通报 Oct. 20, 2016, 43(10): 2129−2137 Microbiology China /wswxtbcn tongbao@ DOI: 10.13344/j.microbiol.china.150688 研究报告 植物乳杆菌 CLP0279 发酵生产低温 SOD 培养基的优化 李晓艳 于爽 窦少华 张庆芳 迟乃玉 王晓辉* ( 116622) 摘 要：【目的】提高植物乳杆菌CLP0279 发酵生产低温超氧化物歧化酶(Superoxide dismutase ， SOD)的能力。【方法】在单因素实验基础上，采用Plackett-Burman (PB)设计、Box-Behnken (BB) 设计和响应面分析法(RSM) ，对发酵培养基进行优化。【结果】植物乳杆菌CLP0279 产低温SOD 最佳发酵培养基(g/L)：玉米粉25.000 ，磷酸二氢钾2.600 ，磷酸氢二钾1.830，硫酸铜0.011 ， 硫酸锌0.014。在最佳培养基条件下产酶活力达到194.82 U/mL，是优化前的1.36 倍。【结论】 通过响应面分析，对植物乳杆菌CLP0279 发酵生产低温 SOD 的培养基进行优化，明显提高了 产酶能力。确定了磷酸氢二钾、硫酸铜和硫酸铵为发酵培养基中影响酶活的3 个关键因子。研 究结果为SOD 的发酵放大提供了依据。 关键词：植物乳杆菌，低温超氧化物歧化酶，发酵培养基，优化 Optimization of fermentation medium for a cold-adapted superoxide dismutase (SOD) produced by Lactobacillus plantarum sp. CLP0279 LI Xiao-Yan YU Shuang DOU Shao-Hua ZHANG Qing-Fang CHI Nai-Yu * WANG Xiao-Hui (College of Life Science and Technology, Dalian University, Dalian, Liaoning 116622, China) Abstract: [Objective] In order to increase the activity of a cold-adapted superoxide dismutase (SOD) from Lactobacillus plantarum sp. CLP0279. [Methods] Based on the single-factor experiment, the fermentation medium for the production of SOD was optimized using Plackett-Burman method, Box-Behnken design and response surface analysis. [Results] The optimal fermentation medium (g/L) was determined as follows: corn meal powder 25.000, KH2PO4 2.600, K HPO 1.830, CuSO ·5H O 0.011, ZnSO ·7H O 0.014. Under these