二阶导数光谱预处理在用FTIRATR方法定量测定葡萄糖-6-磷酸和果糖.PDF

14 1 V ol . 14 N o. 1 2006 2 O ptics and Precisio n Eng ineer ing F eb. 2006 1004-924X 2006) 01- 0001- 07 FTIR/ATR -6--6- 1 2 3, 4 陈洁梅 , 潘 涛 , 陈星旦 1. 暨南大学生命科学技术学院, 广东 广州510632 ; 2. 暨南大学 信息科学技术学院, 广东 广州510632; 3. 暨南大学理工学院, 广东 广州510632; 4. 中国科学院长春光学精密 械与物理研究所, 吉林 长春 1300 33) : FT I R) A T R) , - 6- G6P ) - 6- F6P) PG I) , G 6P- F6P- T r is , G6P- F 6P- T r is , G6P, F6P, T ris 1 0 86 cm- 11 082 cm- 1 1 065 cm- 1 1 0 36 cm- 1 97 8 cm- 1 ) 1 200 ~ 900 cm- 1 ) , 4 , R M SECV ) , , G6P, F 6P , T ris : 傅立叶变换红外光谱; 衰减全反射; 二阶导数光谱; 定量分析 : O 433. 4 , Q493 . 4 , Q 532. 3 : A Application of second derivative spectrum prepares in quantification measuring glucose-6-phosphate and fructose-6-phosphate using a FTIR/ ATR method 1 2 3, 4 CH EN Jie- m ei , PA N T ao , CH EN Xing- dan (1. Collegeof L if e Science and Technology , J inan University , Guangz hou 510632, China; 2. College of Inf ormation Science and Technology , J inan Univ ersity , Guangzhou 5 10632, China; 3. College of Science and ngineering, J inan University , Guangz hou 510632, China; 4. Changchun Institute of Op tics, Fine M echanics and Physics, Chinese A cademy of Sciences, Changchun 130033, China) Abstract: A simult aneou s quant if ication m easurement method of g lucose- 6- phosphat e G6P) and fr uc- t o se- 6- phosphat e F6P) w as developed u sing a F ourier transf orm inf rared FT IR) spect rom et er and at t enuated t ot al ref lect io n AT R) t echniques. Fo r