从生长分化因子-5调控关节软骨形成观察加味当归四逆汤防治膝骨关节.doc

从生长分化因子-5调控关节软骨形成观察加味当归四逆汤防治膝骨关节炎临床研究 韦国雨1 陈清雄1 唐永亮1 李钰威2 （1广西537200；2广东省广州宏宇生物技术有限公司 广州510003） 摘要：目的：通过观察治疗前、后膝骨关节炎患者GDF-5、VEGF及临床症状计分变化，探讨加味当归四逆汤防治膝骨关节炎（KOA）GDF-5、VEGF表达下降；治疗后，两组患者GDF-5、VEGF表达上升，经比较，有统计学差异（P＜0.05）。结论：加味当归四逆汤可能通过上调GDF-5及VEGF表达，发挥缓解KOA的效用，值得推广应用。 关键词：膝骨关节炎；生长分化因子-5，血管内皮细胞生长因子；当归四逆汤 Clinical Study on Modified Dangguisini Decoction Treated with KOA on Regulating Articular Cartilage Formation by Growth Differentiation Factor-5 WEI Guo-yu1, CHEN Qing-xiong1, TANG Yong-liang1, LI Yu-wei2 (1The TCM Hospital in Guiping City, Guiping5372002; 2Hongyu Biological Company of Guangzhou, Guangzhou510003) Abstract: Objective: The mechanism and clinical effect of modified Dangguisini decoction treated with knee osteoarthritis was investigated by observing the expression of GDF-5, VEGF and clinical symptom scoring changes. Methods: It was a randomized controlled trial. 62 Cases of KOA who conformed to the diagnostic criteria, inclusion criteria and exclusion criteria, and randomly divided into two groups according to 1:1 based on treatment sequence. The intervention group was administrated with modified Dangguisini decoction, and control group was gave Xianlinggubao capsule. The clinical symptom scoring changes in detail were recorded before and after treatment for 2 weeks and 4 weeks. The expression of GDF-5 and VEGF were detected by Real-time PCR before and after the treatment. Results: The total curative effect in intervention group: 10 cases were cured, 7 cases had marked effect, 12 cases were effective and 2 cases were ineffective, the total effective rate was 93.54%.However,the effect rate in Control group: 6 cases were clinical cure, 5 cases had marked effect, 8 cases were effective and 12 cases were ineffective, the total effective rate was 61.29%. There was a significant difference between the two group(P＜0.05).Before treatment, there was no significant difference in clinical symptoms scoring between two groups. After 2 weeks treatment, the art