菌悬液稀释倍数与OD_600_的关系实验.pdfVIP

010 3 Vol. 16 N o. 3 OD 600 1, 2 1 徐 凤 , 雷 萍 ( 1. , 710055; 2. , 832003) # : 将实验室从陕北石油污染土壤中分离出来的 S3 菌用牛肉膏液体培 基在摇床中以 30 160 r/m in恒温培 48 h, 离心用磷酸盐缓冲液淋洗 3次后, 用磷酸盐缓冲溶液配制成菌悬 液然后, 用磷酸盐缓冲溶液将菌悬液稀释成不同倍数, 以磷酸盐缓冲溶液作为空白对照, 用 WFJ7200可见分光光度计在 600 nm下测量菌悬液的吸光度得出结论, 溶液稀释倍数和 OD 是 600 乘幂关系, 关系式为y = 1. 402 5x- 0. 9353, R 2 = 0. 998 8 : 菌悬液 稀释倍数 OD600 : 830. 2 : A : 1674- 0254(2010) 03- 0043- 03 The Experim ent on the Relationship between Dilution M ultiples of Bacterial Suspension and OD600 1, 2 1 U Feng , LEIPing ( 1. School ofEnvironment Engineering City Planning, ianUniversity of Architecture andTechnology, ian 710055, China; 2. College ofHydraulic and Constructional Engineering, ShiheziUniversity, injiang 832003, China) # Abstract: The S3 bacteriawhich is isolated from oil- contam inated soil in the northern Shaanxi is culturedw ith beef extract liquid asmedium under the condition of shaker 30 , 160 r/m in for48 hours, centrifugation, washingw ith phosphatebuffer for three tmi es, and in the preparation of bacte rialsuspension by phosphate buffer. Thenwe dilute the bacterialsuspension into different concentra tionmultiplesw ith the phosphate buffer, use phosphate buffer as the blank contrast, and the absor bance