第七章 MST样品标记说明书RED-NHS.pdf

User Manual Cat Nr: L001 Monolith NT™ Protein Labeling Kit RED-NHS Microscale Thermophoresis Grade Kit for 1h labeling of proteins with the RED fluorescent dye NT-647-NHS for use in Microscale Thermophoresis CONTENT AND Monolith NT™ Protein Labeling Kits are shipped on at room temperature . Store at 4°C upon arrival. Each kit contains material sufficient for 4 protein STORAGE labeling reactions. Depending on the amount of protein used, enough material for approximately 1000 Microscale Thermophoresis can be prepared. 4* 25µg NT-647-NHS dye 4* Spin Column A, Buffer Exchange 4* Gravity Flow Column B, Purification 2* Adapter 15 ml Falcons 4* Labeling Buffer Expiry Date: See Kit cover ADDITIONAL MATERIAL  Variable speed benchtop microcentrifuge  1.5 -2 ml microcentrifuge collection tubes REQUIRED  Exchange buffer (buffer of choice e.g. PBS)  100% DMSO  Aq.dest. User Manual Monolith NT™ Protein Labeling Kit V011 Date Feb 2012 1 www.nanotemper.de +49 (0)89 4522895 0 User Manual User Manual Monolith NT™ Protein Labeling Kit V011 Date Feb 2012 2 www.nanotemper.de +49 (0)89 4522895 0 User Manual PROTEIN LABELING PR