人工老化芥菜种子基 因组 DNA 的 ISSR标记.pdfVIP

中国蔬菜 2010 （24 ）：25-30 CHINA VEGETABLES 人工老化芥菜种子基因组 DNA 的 ISSR 标记 1 1 1 1 1 2 宋 明 王鹤冰 孙梓健 王志敏 汤青林 生 1 （西南大学园艺园林学院，南方山地园艺学教育部重点实验室 ，重庆市蔬菜学重点实验室 ，重 2 庆 400715 ；重庆市农业科学院生物工程 心 ，重庆 400055 ） 摘 要：应用 ISSR 分子标记对人工老化的芥菜种子进行DNA 损伤程度的分析，从 18 条 ISSR 引物 中选出6 条 用于老化芥菜种子的研究分析，并发现人工老化处理的芥菜种子随着老化时间的延长，ISSR 扩增出的条带减少、颜色变浅甚至消失 ，表明在种子贮藏中，随着老化程度的加剧 ，DNA 破损是影响种质 资源保存的因素之一。 关键词 ：芥菜 ；人工老化 ；DNA ；ISSR 图分类号 ：S637 文献标识码 ：A 文章编号 ：1000-6346 （2010 ）24-0025-06 Studies on ISSR Marker of Artificial Aging Mustard Seed Genomic DNA 1 1 1 1 1 2 SONG Ming , WA NG He-bing , SUN Zi-j ian , WANG Zhi-min , TA NG Qing-lin , ZHANG Sheng 1 （Colleg e of H orticulture and Landscape A rchitecture, Southwest University, K ey L aboratory of H orticulture Science f or Southern M ountainous R eg ions, M inistry of Education, K ey L ab in Olericulture of Chongq ing, Chongq ing 400715, China; 2B ioeng ineering Centers, Chongqing A cademy of Ag ricultural Sciences, Chongqing 400055, China ） Abstract ：ISSR molecular marker was applied to analyze the DNA damaging degree of artificial aging muster seed. Six primers suitable for studying aging mustard （Brassica j uncea Coss. ）seeds were selected from 18 primers. We found that along with the prolongation of aging time of muster seed w ith artificial aging treatment, the numbers of ISSR amplified bands were decreased and their color became light and even faded away. This indicated that during the seed storage, along w ith the intensification of aging, DNA damages was one of the factors influencing conservation of germplasm resources. Key words ：Brassica j uncea Coss.; Artificial aging; DNA ; ISSR 植物种质资源是物种进化、遗传学研究及植物育种的