The complete genome sequence of Escherichia coli K-12-英文文献.pdf

ARTICLE The Complete Genome Sequence of Escherichia coli K-12 Frederick R. Blattner,* Guy Plunkett III,* Craig A. Bloch, Nicole T. Perna, Valerie Burland, Monica Riley, Julio Collado-Vides, Jeremy D. Glasner, Christopher K. Rode, George F. Mayhew, Jason Gregor, Nelson Wayne Davis, Heather A. Kirkpatrick, Michael A. Goeden, Debra J. Rose, Bob Mau, Ying Shao The 4,639,221–base pair sequence of Escherichia coli K-12 is presented. Of 4288 The first 1.92 Mb ( 13, 14), positions protein-coding genes annotated, 38 percent have no attributed function. Comparison 2,686,777 to 4,639,221 [in base pairs (bp)], with five other sequenced microbes reveals ubiquitous as well as narrowly distributed was sequenced from our overlapping set of gene families; many families of similar genes within E. coli are also evident. The largest 15- to 20-kb MG1655 lambda clones ( 15) family of paralogous proteins contains 80 ABC transporters. The genome as a whole is by means of radioactive chemistry and was strikingly organized with respect to the local direction of replication; guanines, oligo- deposited in GenBank between 1992 and nucleotides possibly related to replication and recombination, and most genes are so 1995. Subsequently, we switched to dye- 6 1 oriented. The genome also contains insertion sequence (IS) elements, phage remnants, terminator fluorescence sequencing (Ap- 0 2