利用Minitab优化耐高温淀粉酶发酵培养条件.pdfVIP

Vol.41 No.5 中南大学学报(自然科学版) 第41 卷第5 期 Journal of Central South University (Science and Technology) Oct. 2010 2010 年 10 月 利用 Minitab 优化耐高温淀粉酶发酵培养条件 赵伟，陈艇，刘惰，冯婷婷，周洪波 (中南大学资源加工与生物工程学院，湖南长沙， 410083) 摘 要:利用 Minitab 软件中的 Plackett-Burman 实验设计和响应四分析法，对耐高温 α·淀粉酶产1:菌 Bacillus subtilis C1 的发酵培养条件进行二优化。在单次单剧手法得出的适宜条件基础上，踪介考虑所有影响国浆， 对培养条件进行统计分析，得到 Bacillus subtilis Cl 产耐高跑 φ能粉酶的最佳培养条件。研究结果表明t 优化培 养基的组成(200 mL)为数皮 2.1 6 g，棉柏粉 1.98 g，酵母粉 0.40 g. NaCI 1.00 g. CaCh 0.40 g. 淀粉0.10 g. 培养 基优化后的酶附为 2329U旭L，约是培养基优化前酶活的 12.55 倍，酶拍得到恩将:提高。 提键词: Plackett.也 中阻分古怪号: Q556+.2 文献标志响:A 文章编号: 1672-7207(2010)05… 1652… 06 Optimization of medium for thermostableα酬amylase fermentation using Minitab ZHAO Wei , CHEN Chen, LIU Qian, FENG Ting-ting, ZHOU Hong-bo (School of Mínerals Processing and Bioengineering, Central South University , Changsha 410083 , China) Abstract: The optimum medium for Bacillus subtilis C 1 was systematically studied with Minitab so企ware. On the basis of single 岛ctor experiments, the príme factors affecting enzyme activity were selected by means of Plackett-Burman desígn. And then , the factors were optimized by response surface analysis. The results show that the optimal medium composition in 200 mL culture medium isωfollows: wheat bran 2 .16 g, cotton seed meal 1.98 g, yeast extract 0.40 g, NaCl 1.00 g, CaCl2 0.40 g, starch 0.1 0 g. Enzyme activity under the optimal culture conditions is 2 329 U/mL, whích ís 12.55 times ofthat ofthe original medium. Key words: Plackett心urman de