高效液相色谱法测定蓝莓果浆中糖的组成和含量.pdfVIP

※分析检测 食品科学 2009, Vol. 30, No. 06 229 张英春，董爱军，杨 鑫* ，张 华，马 莺 (哈尔滨工业大学食品科学与工程学院，黑龙江哈尔滨 150090) 目的：建立高效液相色谱法测定蓝莓果浆中糖的组成和含量的检测方法。方法：超声波水提样品中的糖 类，色谱柱为Agilent Zorbax carbohydrate 柱(4.6 ×250mm，5 μm) ，流动相为乙腈- 水(75:25，Ｖ:Ｖ)，流速为1ml/min。 结果：糖类浓度在0.5～10mg/ml 内呈现良好的线性关系，相关系数在0.9989(n=5) 以上；精密度实验RSD 为0.36%～ 2.18%(n=5) ；平均回收率为91.36% ～101.34%。结论：该方法简便快速，结果准确可靠，为测定野生蓝莓果浆 中糖组分提供了一种高效快速的测定方法。 蓝莓果浆；果糖；葡萄糖；蔗糖；高效液相色谱法 Determination of Saccharide Components and Contents in Blueberry Juice by HPLC ZHANG Ying-chun ，DONG Ai-jun ，YANG Xin* ，ZHANG Hua ，MA Ying (College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China) Abstract ：Objective: To set up an examination method for determining saccharides in blueberry juice by HPLC. Method: Saccharides were extracted from samples with water using ultrasonic, and then their components and contents were determined by HPLC. Agilent Zorbax carbohydrate (4.6 × 250 mm, 5 μm) column was adopted, mobile phase was acetonitrile-water (75: 25, V:V), and the flow rate was 1 ml/min. Results: The calibration curve of saccharide concentrations is well linear in the range of 0.5 to 10 mg/ml, the correlation coefficient is over 0.9989 (n=5), the RSD is 0.36% to 2.18% (n=5) in precision experiment, and the average recovery rate is 91.36% to 101.34%. Conclusion: This method is convenient, rapid and effective for separation and quantitative analysis of saccharides in blueberry juice. Key words ：blueberry juice ；fructose ；glucose ；sucrose ；HPLC 中图分类号：O652.63 文献标识码：A 文章编号：1002-6630(2009)06-0229-03 蓝莓(Ericaceae vaccinium ，Blueberry)属于越橘科 单糖、低聚糖和多元醇，因此近年来用液相色谱法测 乌饭树属的落叶性或常绿性的灌木或半乔木果树。蓝莓 定食品中糖的组成和含量得到了快速发展[3-