稻曲病菌不同颜色厚垣孢子超微结构比较.pdfVIP

植物病理学报 ACTAPHYTOPATHOLOGICASINICA 40(5)：538—542(2010) 一旷“报简研究一一一 一 稻 曲病菌不 同颜色厚垣孢子超微结构 比较 燕玮婷 ，刘二明 ，邓林伟 ，黄红梅 ， 刘东波。，吕建林 ，奉光平 ，毛 莹 (湖南农 、l夫 ：，1：物安全科学技术学院，长沙 410128； 湖南农 、1大学，t物科学技术学院，长沙 410128 湖南农、夫学分析测试中t2,，长沙410128) UItrastructurecomparisonofchlamydosporeswithdifferentcolorofUstilaginoidea virens YAN Wei—ting。，LIU Er—uring’，DENG Lin—wei，HUANG Hong—mei，LIU Dong—bo。，LV Jian—lin。，FENG Guang—ping ，MAO Ying (。CollegeofBio—safetyScienceandTechnology，HunanAgricultural University，Changsha410128，China； CollegeofBioscience andBiotechnology，Hunan AgriculturalUniversity，Changsha 410128，China；CenterofAnalyticalService，HunanAgriculturalUniversity，Changsha410128，China) Abstract：Thesurfaceandinternalstructuredifferenceofyellow andblackdormantchlamydosporesofUsti— laginoideavirensonmid—andlate—seasonricewereexaminedbyscanningelectronmicroscopy (SEM)and transmissionelectronmicroscopy(TEM)．SEM revealedthatthesphericalyellowandblackchlamydospores hadprominentspines，withthediametersfrom 3to6txm and4to6 ．／zm ，respectively．Thespineswere200— 700 nm and400—1100nm longinyellow andblackchlamydospores，respectively．Varianceanalysisshowed thattherewasno difference in diameterofthe yellow chlamydosporesbutsignificantdifference among the blackonescollectedrfom bothricecroppingtypes．Similarly，therewasnodifferenceinlengthofthespines onthesamecolorchlamydosporesbutextremelydifferentbetweentheyellow andblackchlamydosporescol— lectedfrom thetwocroppingtypes．TEM revealedthatthecellwallofbothcolorchlamydosporeswerecom— posedoftwolayers，thethickendosporium andthinexosporium ，andthethicknessoftheformerlayerofthe blackchlamydosporeswasnearlytwo timesasthatoftheyellow ones．Theorganellesinyellow chlamydo— sporessuchasthenucleuswereobviouslyvisible，butinvisibleinblackones．Thecytoplasm ofblackchla— mydosporeswasoccupiedbyabiglipidglobule．Thiss