1.正交模拟法研究护骨胶囊各单味药与复方药效-贾欢欢.docx

正交模拟法研究护骨胶囊各单味药及复方药效贾欢欢1，高建林1，陈珺1，卢丽1，陆幸妍1，杨国柱1，曾雯2，秦中华2，李英彬1，万超3，李青南1*( 1. 广东药学院，生命科学与生物制药学院，新药功能研究中心，广州510006;2. 东莞万成制药有限公司，广东东莞523040; 3. 香港中文大学医学院，生物医学学院，干细胞与再生医学中心，香港)［摘要］目的: 应用正交模拟法分析护骨胶囊( HG) 各药味对该复方药效的贡献值，并对该方不同组合的复方进行药效模拟。 方法:采用正交模拟法，将HG 10 个药味按照设计的正交表分为13 个组( 第13 组为阴性对照组) ，通过血清药理学实验得出相应含药血清，采用CCK-8 法检测各组血清对成骨细胞( Ob) 增殖的影响。DAS3. 0 软件处理数据，分析各药味的药效贡献值并对复方药效进行模拟。结果: 以Ob 增殖为指标，经正交模拟法分析，确定HG 各药味在复方中的药效贡献值顺序为: 淫羊藿＞骨碎补＞巴戟天＞山药＞龟甲＞当归＞熟地黄＞续断＞杜仲＞制首乌; 以Ob 增殖为指标，对复方药效进行模拟研究，结果显示27 个模拟组方预测药效值明显优于阴性对照组，而且原方最好，4 个模拟组方接近于原方。结论:正交模拟法分析HG，确定了HG 各药味在复方中的药效贡献值，并发现多个组方的模拟药效与原方接近。［关键词］ 正交模拟法; 护骨胶囊; 成骨细胞; 药效贡献值; 模拟药效［中图分类号］R285. 5［文献标识码］A［文章编号］1005-9903( 2011) 15-0161-05Pharmacodynamic Contributed Values of Composition and PharmacodynamicSimulation of Compound Hugu Capsules by Orthogonal Simulation MethodJIA Huan-huan1，GAO Jian-lin1 ，CHEN Jun1，LU Li1，LU Xing-yan1，YANG Guo-zhu1 ，ZENG Zhao-li2 ，ZENG Wen2 ，QIN Zhong-hua2，LI Ying-bin1，WAN Chao3，LI Qing-nan1*( 1. School of Life Science and Pharmacopedia，Guangdong Pharmaceutical Universty，Guangzhou 510006，China;2. Dongguan Wancheng Pharmacopedia Company Limited，Dongguang 523040，China;3. Conter of Stem Cell and Regenerative Medicine，School of Biomedical Sciences，Faculty of Medicine，The Chinese University of Hong Kong，Shatin，Hong Kong SAR，China)［Abstract］Objective: To analysis pharmacodynamic contributed values of composition andpharmacodynamic simulation of compound Hugu capsules ( HG) ．Method: Ten components of HG were divided into13 groups ( group 13 for negative control group) by orthogonal simulation method，the drug serum was gotten by theserum pharmacological experiments and CCK-8 method was adopted to detect osteoblast ( Ob ) proliferation．Pharmacodynamic contributed values and pharmacodynamic simulation of compound were analysis by DAS3.0 software．Result:The proliferation of Ob as a index，a sequence of pharmacodynamiccontributed values were foundby orthogonal simulation method: Epimedii Folium ＞ Drynariae Rhizoma ＞ Morindae Offici