油桐种子脂肪酸代谢途径基因调控分析介绍.PDFVIP

第 32 卷 第 3 期 经 济 林 研 究 Vol. 32 No.3 2014 年 9 月 Nonwood Forest Research Sep. 2014 油桐种子脂肪酸代谢途径基因调控分析 周乃富a，b ，谭晓风 a，b ，黄丽媛 a，b ，龙洪旭 a，b （中南林业科技大学a. 经济林培育与保护省部共建教育部重点实验室； b. 经济林育种与栽培国家林业局重点实验室，湖南 长沙4 10004 ） 摘 要：为了探明油桐脂肪酸代谢机理的理论基础，以油桐优 良品种葡萄桐的种子为试材，构建其转录组数据库， 综合分析了转录组和表达谱的数据库，得到了与油桐脂肪酸代谢相关的非冗余基因序列共 83 条，涉及主要酶基 因 10 种；同时以KEGG 数据库为参考，对油桐脂肪酸代谢途径进行了分析，绘制了油桐脂肪酸代谢的路径图， 揭示了油桐脂肪酸代谢途径中关键酶基因调控及催化脂肪酸代谢的基本规律。 关键词：油桐；转录组；脂肪酸代谢；基因表达 中图分类号：S603.2 ；S794.3 文献标志码：A 文章编号：1003—8981(2014)03—0016—06 Gene regulation of fatty acid metabolism pathways in Vernicia fordii seeds ZHOU Nai-fua,b , TAN Xiao-fenga,b , HUANG Li-yuana,b , LONG Hong-xua,b (a. The Key Lab of Cultivation and Protection for Non-wood Forest Trees of Education Ministry; b . The Key Lab of Non-Wood Forest Product of State Forestry Administration, Central South University of Forestry Technology, Changsha 4 10004, Hunan, China) Abstract: In order to ascertain theoretical basis of fatty acid metabolism mechanism in Vernicia fordii, taking seeds of a fine variety ‘Grape Tong’ as materials, its transcriptome database was established, the transcriptome and expression spectrum database were comprehensively analyzed, total 83 non-redundant gene sequences were found, which involved in fatty acid metabolism in V. fordii and 10 main enzyme genes. Taking KEGG database as a reference, the fatty acid metabolism pathways in V. fordii were analyzed, and its pathway graph was drawn, which revealed the basic rules of key enzyme genes regulation and catalytic fatty acid metabolism in fatty acid metabolism pathways in V. fordii. Key w