堆肥作为微生物菌剂载体的研究.pdfVIP

农业环境科学学报 2010,29(7):1382-1387 Journal of Agro-Environment Science 堆肥作为微生物菌剂载体的研究 张志红，李华兴，冯 宏，赵兰凤，李敏清，胡 伟 （华南农业大学资源环境学院，广州 510642） 摘 要：以自然风干的堆肥为载体，吸附3 株功能芽胞细菌液体菌剂制成不同生物肥料，通过不同时间取样比较堆肥、有机无机肥 和生物有机肥以及生物复混肥中功能芽胞细菌和普通微生物数量以及pH 等指标变化，探讨堆肥作为载体生产生物肥料的可行 性。研究结果表明，经过自然风干的堆肥与蛭石比较，吸附液体微生物菌剂后无论外观、手感还是功能芽胞细菌死亡率，差异均不 大。含水量小于15%堆肥吸附液体菌剂比例为6% 比较合适，吸附比例高时，生物肥料含水量和pH 较高，影响保存效果。生物有机 8 -1 8 -1 肥和生物复混肥保存6 个月时，3 株功能芽胞细菌总数分别为0.59×10 CFU ·g 和0.38×10 CFU ·g ，依然可达到农业行业标准要 求。生物有机肥中功能芽胞细菌数量最高，生物复混肥集合了三大肥料优点，堆肥中普通微生物数量和多样性最高。完全腐熟的堆 肥经过自然风干后可作为微生物菌剂载体。 关键词：堆肥；载体；微生物菌剂；生物有机肥；生物复混肥 中图分类号：S141.4 文献标志码：A 文章编号：1672- 2043(2010)07- 1382- 06 Compost as a Carrier for Microbial Inoculants ZHANG Zhi-hong, LI Hua-xing, FENG Hong, ZHAO Lan-feng, LI Min-qing, HU Wei （College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China） Abstract ：The work mainly studied whether air-dried compost （utilizing livestock or poultry manure and spent mushroom substrate as composting materials）could be used as carrier for liquid microbial inoculants. Three functional Bacillus strains （Bacillus megaterium, Bacil－ lus mucilaginosus and Bacillus subtilis）were used to construct microbial-organic fertilizer （MOF）and microbial-compound fertilizer （MCF）. The application effects of the different bio-fertilizers including compost （C）, organic-inorganic fertilizer （OIF）, MOF and MCF were tested. The results showed that air-dried compost had no significant difference compared with vermiculite in appearance, touch-feeling , bacterial mortality after adsorbing microbial inoculants. When moisture of compost less than 15%, about 6% （v/w）adsorption proportion of liquid mi－ crobial inoculants to compost was better. The higher proportion of liquid microbial inoculants to compost caused higher moistur