miRNA讲稿(徐和学生的用).ppt

miRNA简介;miRNA定义： ;miRNA定义：;miRNA*定义;;;;miRNA和siRNA区别;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;;;;;一般认为．siRNA 的特异性很高． 不会发生结合于非目标mR N A 的脱靶情况(off -targeting) 。但是． 研究发现miRNA的结合能力存在摆动． 1 个miRNA可抑制多个mRNA 的功能。研究人员预测在miRNA 靶点药物开发方面,这一特点具有重要意义,因此miRNA的作用效果可能更高，但是作用机理更复杂，对基因调控的网络作用往往难以研究清楚。;miRNA作用方式;张辰宇:吃什么补什么;张辰宇:微小RNA的人体之旅;;;miRNA网站;;;;;;;;;;;;;;;;;m i R N A与肿瘤研究;m i R N A与肿瘤研究;m i R N A与肿瘤研究;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;A549/T与A549细胞miRNA芯片的qPCR验证;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;miRNA在肿瘤治疗中的希望;早期发现的miRNA;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;作为肿瘤抑制因子;作用机理;作为癌基因;作为肿瘤诊断;经典的miRNA合成途径;作用机理;推测;mirtron途径 ;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;疾病治疗;疾病治疗 ;反义技术： miR21: 5’-UAGCUUAUCAGACUGAUGUUGA-3’ 反义DNA:5’-TCAACATCAGTCTGATAAGCTA-3’ 硫代、氟代、甲基化等修饰，L2000转染。动物实验。同以往的反义寡核苷酸技术类似。 ;反义药物;首个在人体中试验的miRNA药物;miRNA药物;前景;同Bcl2相关的目前进行比较多的研究是过表达miR-181,145,15a,15b,16,34,或用反义抑制21 ;肿瘤耐药性;肿瘤耐药性;;cardiac fibroblasts directly to cardiomyocytesCirculation Research published online April 26, 2012;研究小组成功利用诱导性多能干细胞（iPS细胞）人工制成癌干细胞并在小鼠身上得到验证;研究一般方法;抑制相关基因表达;siRNA与miRNA药物不同;Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd.;Evaluation only. Created with Aspose.Slides for .NET 3.5 Clie