《生命科学研究》论文修改范例稿幻灯片.docVIP

《生命科学研究》论文修改范例稿 人参皂苷Rg1对丙二醛抑制小鼠间充质干细胞成骨分化的 保护作用 黄思洋1，，1，，李 烨1* ，中国湖南 长沙 410081；2. 湖南省长沙市第一中学 中国湖南 长沙 410005；3. 湖南科技职业学院，中国湖南 长沙 410004) 摘要：人参皂苷Rg1ginsenoside Rg1，GS-Rg1对丙二醛alondialdehyde，MDA制小鼠间充质干细胞esenchymal stem cells，MSCs成骨分化的保护作用机制在MSC成骨诱导培养下，以不同剂量10 mg/L、50 mg/L、100 mg/LGS-Rg1对成骨诱导培养小鼠骨髓MSC预处理24，然后用丙二醛，on Kossa染色茜素红染色检测钙结节形成碱性磷酸酶活性alkaline phosphatase，ALP)活性，利用Q-RT-PCR和Westen-blot检测ALP)和Runt-related transcription factor 2，Runx2)的mRNA及蛋白表达GS-Rg1能够增加钙结节形成及碱性磷酸酶活性，上调ALP和Runx2表达GS-Rg1对MDA羰基应激MSCs的成骨分化具有保护作用。 关键词：人参皂苷Rg1；间充质细胞；丙二醛；成骨分化Q955 文献标识码：A Protective Effect of Ginsenoside Rg1 Against MDA-Suppressed Osteogenic Differentiation of Mesenchymal Stem Cells HUANG Si-yang1, CHEN Ji-yao2, WANG Yi-an1, ZHANG Jun3, LI Ye1* (1. College of Life Sciences, Hunan Normal University, Changsha 410081，Hunan, ChinaHunan, China; 3.Hunan Vocational College of Science Technology, Changsha 410004, Hunan, China) Abstract: Cytoprotective effects of ginsenoside Rg1 (GS-Rg1) against malondialdehyde (MDA)-suppressed osteogenic differentiation of mesenchymal stem cells (MSC) and the possible mechanisms were investigated in vitro. After osteogenic differentiation of MSCs was induced by estrogenic medium, MSCs were pretreated with GS-Rg1 at different doses ( 10 mg/L, 50 mg/L, 100 mg/L) for 24 h and then treated with MDA (0.1 mmol/L) for 24 h. During the research, the formation of mineralized nodule and the activity of alkaline phosphatase (ATP) were examined. The mRNAs and protein expression of Runx2 and ALP were analyzed by Q-RT-PCR and Western-blot, respectively. The results showed that pretreatment of GS-Rg1 increased calcium nodule formation and ALP activity, and up-regulated expression of ALP and Runx2, which indicated that GS-Rg1 protected MSCs from MDA-suppressed osteogenic differentiation. The upregulation of ALP and Runx2 by GS-Rg1 may contribute to the protective role in osteogenic differentiation of MSCs. Key words: ginsenoside Rg1 (GS-Rg1); mesenchymal stem cells (M