植物机能を活用した高度モノ作り基盘技术开发／植物利用高付加似值 ....ppt

* 　 Honoo Satake Metabolic engineering of lignan biosynthesis in Forsythia O H O C H 3 O H H O O O H H O H O C H 3 O C H 3 O H O O H C H 3 coniferyl alcohol x 2 (+)-pinoresinol H O O H H O H O C H 3 O C H 3 O H (+)-lariciresinol (-)-matairesinol H O O H H 3 C O H H O C H 3 O O Forsythia (+)-pinoresinol glucoside H O O O H H O -Glc O C H 3 O C H 3 (-)-podophyllotoxin Linum (-)-matairesinol H O O H H 3 C O H H O C H 3 O O O O H H O O O O O O H H O H O C H 3 O O O O H H O O O O (+)-piperitol (+)-sesamin O H (+)-sesaminol Sesamum Lignans are species-unique plant secondary compounds Sesamin: A sesame lignan exerting beneficial actions on humans A furofuran lignan Most abundantly contained in sesame seeds (~0.5% of sesame oil) O O H H O O O O (+)-sesamin O O H H O H O C H 3 O O (+)-piperitol H O O O H H O H O C H 3 O C H 3 (+)-pinoresinol CYP81Q CYP81Q Sesamin is biosynthesized from pinoresinol via the formation of two methylenedioxy bridges by a unique enzyme, CYP81Q. Sesamin has been shown to exert a wide variety of biological effects: such as anti-oxidative activity, anti-hypertensive activity, and protection of the liver from alcohol. Sesamin has become commercially available as a new health supplement. The demand for sesamin has been markedly increasing. Metabolic engineering of lignan biosynthesis (=Generation of sesamin-producing transgenic plants) ? Very small amounts: sesamin comprises at most 0.5% component of sesame oil, which most abundantly contains sesamin. ?Sesame seeds are cultivated only once every year. ? Japan imports 99% of its sesame seeds. Convert “agricultural production” into industrial production using a transgenic plant in a “plant factory” Low efficiency in acquisition of sesamin via extraction from sesame seeds Which plant is the best transgenic host for sesamin production? Forsythia spp. as a transgenic host for sesamin production ?Perennial woody plants ? Their leaves and fruits are used as Chinese medicines because they conta