健骨颗粒对骨质疏松模型大鼠垂体-肾上腺轴影响.PDF

中国骨伤2004 年 1 月第 17 卷第 1 期 C ina J Ort op Trauma, Jan. 2004, Vol. 17, No. 1 # 19 # # # 健骨颗粒对骨质疏松模型大鼠垂体- 肾上 腺轴的影响 1 2 1 1 林燕萍, 周瑞祥 , 马建华, 黄美雅 ( 11 福建中医学院骨伤系, 福建 福州 350003;21 福建医科大学 础医学院) :从垂体- 肾上腺轴角度探讨健骨颗粒治疗Ñ型原发性骨质疏松症的作用机制。: 通过切除SD 大鼠双侧卵巢建立Ñ型原发性骨质疏松模型, 随机分成 3 组, 分别喂服健骨颗粒、骨松宝 和生理盐水。用药12 周后处死取材, 检测血清皮质醇(COR) 浓度, 取脑垂体行ACTH 细胞免疫化学染 色( S- P 法) 并形态计量, 肾上腺行HE 染色。:切除卵巢 12 周后大鼠出现骨质疏松, 血清COR 浓 度以及垂体ACTH 阳性细胞数和染色面积均明显高于假手术组(行腹部切口, 但不切除卵巢) 。用药 12 周后健骨颗粒组血清COR 水平、垂体ACTH 阳性细胞数和染色面积均较生理盐水组降低或减少。 :补肾健脾中药健骨颗粒可有效调节去卵巢骨质疏松模型大鼠垂体- 肾上腺轴的病理变化和功能状 态, 抑制雌激素水平降低后的异常骨吸收。 骨质疏松, 绝经后; 垂体; 肾上腺; 中草药 Effect of Jiangu granule on thepituitary2 adrenal gland axis of ovariectomized rats LIN Yan2ping , ZH OU Rui2xiang , MA Jian2hua, H UANG Mei2ya. Department of Orthopae ics Traumatology of Fuj ian TCM ( Fuj ian Fuzhou, 350003, China) Abstract Objective:To investigate t e effect of Jiangu granule on t e primary osteoporosis( POP) in t e view of t e pituitary2 adrenal gland axis. Methods:POP animal model type I was made by ovariectomized in SD rats, W ic were randomly divided into Jiangu granule group, Gusongbao group and saline group. T e animals were killed at 12 weeks after treatment. T e levels of serum COR were measured. T e ACTH cells in pituitary were stained wit immunocytoc emical met od and observed wit morp ometry. T e adrenal gland was stained wit HE.Results: Osteoporosis occurred at 12 weeks after ovariectomy. T e level of t e serum COR and t e number of t e positive ACTH cells were ig er t an s am2 operation group. After 12 weeks of treat2 ment, t e levels of t e serum COR and t e numbers of t e posit ive ACTH cells in Jiangu granule groups were lower t an t e saline group. Conclusion:Jiangu granule can adjust t e functional statement of t e pituitary2 a2 drenal gland axis of o