反相高效液相色谱法测定痹痛消丸中士的宁和马钱子碱的含量.PDF

160 2006 20 3 冉卫兵( , 050081) : 建立痹痛消丸中士的宁和马钱子碱的反相高效液相分析法采用 Diamonsil C18 色谱柱; 流动相 为 腈- 甲醇- 005mo l L- 1 KH 2 P O4 溶液- 三 胺 ( 25 19 55 1) , 磷酸调pH 至35; 流速1ml min- 1 ; 检测波长254nm士的宁在 1549~ 18588g ml- 1 ( = 09999) 马钱子碱在 505~ 6060g m l- 1 ( = 09998) 范围内呈良好的线性关系; 士的宁平均回收率为9842% , R SD 为114% ; 马钱子 碱平均回收率为10030% , RS D 为107% 本方法准确可靠, 简便灵敏, 重现性好, 可用于痹痛消丸中 士的宁和马钱子碱的质量控制 : 痹痛消丸; 士的宁; 马钱子碱; 反相高效液相色谱法 : R9272 : A : 1002- 7777 ( 2006) 03- 0160- 03 Determination of Strychnine and Brucine in Bitongxiao Pills by RP- HPLC Ran Weibing ( H ebei A m ed P olice H eadqua t e s H ospital, Shijiazhuang, 050081) ABSTRACT An RP - H P L C method w as est ablished f o t he dete minatio n of st ychnine and b ucine in - 1 bit ongx iao pills. A Diam onsil C18 co lum n w as used w it h aceto nit ile- met hanol- 005mo l L KH 2 P O4 solut ion- t iethylam ine ( 25 19 55 1) as m obile phase and pH v alue w as adjust ed to 35. T he f low ate w as 1ml min- 1 and detect ion w aveleng t h w as 254nm. T he linea elat ionship w as g ood w hen the cont ent of st ychnine w as 1549~ 18588g ml- 1 ( = 09999) and b ucine w as 505~ 6060g m l- 1 ( = 09998) . T he ave age ecove y and RS D w e e 9842% and 114% fo st ychnine and 10030% and 107% fo b ucine espect ively. T he m et hod w as accu ate and eliable, simple, sensit iv e and ep oducible. It could be used fo quality cont ol f o st y chnine and b ucine in Bit ongx iao pills. KE WORDS Bito ng xiao Pills; st ychnine; b ucine; RP- H PL C 2 , 21 ( RP - H PL C) : Diamo nsil C18 ( 46 ! 250mm, ,