栉孔扇贝一种球形病毒的分离纯化及其超微结构观察.PDFVIP

26 2 Vol. 26, No . 2 2002 4 JOURNAL OF FISHERIES OF CHINA Apr. , 2002 文章编号: 1000- 0615( 2002) 02- 0180- 05 # # 1 1 1 1 2 王崇明, 王秀华 , 宋晓玲 , 黄 , 宋微波 ( 1. , 266071; 2. , 266003) : ; ; ; : S 44.4 : A Purification and ultrastructure of a spherical virus in cultured scallop Chlamysf arreri 1 1 1 1 2 WANG Chong-ming , WANG Xiu-hua , SONG Xiao-ling , HUANG Jie , SONG Wei-bo (1. Key Laboratoryf or Sustainable Utilization of Marine Fisheries Resource by the Ministry of Agriculture Yellow Sea Fisheries Research Institute, ingdao 266071, China; 2. Fisheries College of Ocean University of ingdao, ingdao 266003, China) Abstract: Electron microscopic examinations of ultrathin sections reveal the presence of spherical virus-like particles in the digestive gland, kidney, and the intestine of moribund scallop Chlamysf arreri collected during a severe mortality in Jiaozhou Bay ( Qingdao, China) in 2000. The virions are approximately 130 to 170 nm in diameter and have a bilaminal envelope, while the nucleocapsids are 0 - 140 nm. The virus occurs and distributes in the cytoplasmic vesicles of infected cells, and it is arranged in a scattered form without occluding protein. The viral particles are consistent in size and morphology in preparations derived from the isolation and also from ultrathin sections. The spikes of envelope are clearly observed with negative staining. By the same methods, t