锯缘青蟹精子入卵过程的扫描电镜观察 - 水产学报.PDFVIP

　第24 卷第5期 水　产　学　报 Vol.24, No.5　 2000 年10 月　　　 　　　　　　　　 　 　　　　　 　　　　 Oct., 2000　 JOURNA OF FISHERIES OF CHINA 文章编号:1000-0615(2000)05-0403-05 锯缘青蟹精子入卵过程的扫描电镜观察 康现江, 　李少菁, 　王桂忠 (厦门大学海洋学系, 福建 厦门　361005) 摘要:利 扫描电镜详细观察了锯缘青蟹精子入卵的过程。精子以其核突起附着在卵膜上, 并迅速发生顶 体反应。顶体反应时, 顶体囊外翻, 顶体管前伸, 精子核辐射臂收缩, 并拖至顶体囊的后部。顶体管迅速 穿过卵黄膜, 携带核物质一同进入卵子。锯缘青蟹为多精着卵, 数精入卵。本文同时探讨了精子顶体反应 机制以及受精过程卵子的作 。 关键词:锯缘青蟹;受精;扫描电镜 中国科分类号:S917　　　文献标识码:A Observation on the process of sperm penetration into egg of Scylla serrata with a scanning electron microscope KANG Xian-jiang, 　I Shao-jing, 　WANG Gui-zhong (Dep artment of Oceanograp hy , Xiamen University , Xiamen 　361005, Ch ina ) Abstract:The process of sperm penetration into egg of S cy lla serrata was examined with a scanning electron microscope (SEM)in this paper.The sperm contacts with vitelline membrane by its radial arms of the nucleus, and undergoes acrosome reaction immediately.During the process of acrosome reaction, the reversion of acrosonal vesicle, the forword-extending of the acrosomal tubule and the contraction of the radial arms were observed.The nuclear cup separates itself from the acrosome and is pull at the back of the acrosonal vesicle reversed.While the acrosome tubule passes through the egg membranes, the nucleus was carried into the egg. Because S .serrata has no micropyle, in the process of fertilization, a great number of sperms attach themselves to the egg and then several of them penetrate into the egg.The mechanism of the acrosome reaction and the action of the egg during the fertilization were discussed in this paper. Key words:Scylla serrata ;insemination;scanning electron mi