海水小球藻絮凝采收与藻油提取工艺的初步试验分析.pdfVIP

Abstract Abstract With the advantages of high growth speed, utilization rate of light energy and lipid content, microalgae is an important renewable resource. But because of its minuscule cell and high moisture content, the microalgae harvesting and lipid extraction is of great difficulty, and also become the bottleneck of industrial production of the microalgae energy. At present, research reported at home and abroad mainly focuses microalgae lipid extracted from microalgae powder, but reports about the direct use of wet paste for lipid extraction are relatively few. Marine chlorella vulgaris was taken as raw material, and experiments on technology of microalgae flocculation and lipid extraction were carried out in order to provide important references for the biodiesel synthetic process. Firstly, flocculation harvesting experiment of the chlorella vulgaris was conducted, the results showed that compared with aluminum sulfate and ferric chloride, flocculation harvest with calcium hydroxide had lower cost, higher recovery rate and the flocculant could be easily removed by blowing CO2 into the culture water. Results of single factor experiment and optimization of response surface analysis showed that the recovery rate of chlorella vulgaris reached to 92.2% when the flocculation time, dosage of calcium hydroxide, and pH of culture fluid were respectively maintained at 77 min, 0.7 g/L and 9.3, and the microalgae cells were complete, so it was a highly efficient and low-cost concentration method of chlorella vulgaris. Using different solvents to extract microalgae lipid, the experimental results showed that t