猪尿冻干粉中盐酸克伦特罗标准物质的研制.pdfVIP

中文摘要 盐酸克伦特罗俗称 “瘦肉精”，是一种人工合成β- 肾上腺素受体激动剂，在 促进动物生长、提高饲料转化率和利用率以及产品品质等方面确实起到了十分显 著的作用。然而，一连串因食用含“瘦肉精”的动物源食品中毒事件发生后，包括 我国在内世界上许多国家明文规定不得在养殖业中使用盐酸克伦特罗。但是由于 经济利益的驱动、检测难度大，加之人们对其饲用的危害性认识不足，各国违法 滥用现象仍较普遍。目前，活体样本宰前尿检是保证动物源食品安全监管的一个 有效手段，盐酸克伦特罗残留检验已成为生猪屠宰前的必检项目之一。 论文研究了猪尿冻干粉中盐酸克伦特罗标准物质的制备及其定值方法，并以 健康安全的猪尿作为空白基体，利用添加的方法，研制了一套五种浓度水平的猪 尿冻干粉中盐酸克伦特罗(CLB)标准物质。经过均匀性和稳定性检验，由8 家实 验室采用液相色谱- 串联质谱法对该套标准物质进行协同定值，并进行了不确定 度评估。结果表明该套标准物质均匀性良好，在4 ℃条件下保存稳定性在1 年以 上。标准物质中盐酸克伦特罗的含量及不确定度分别为：小于 0.10 ng/mL(空白 猪尿冻干粉) 、(0.65±0.15)ng/mL、(1.28±0.44)ng/mL、(3.78±0.46)ng/mL 和(11.48 ±1.72) ng/mL(k=2) 。该套标准物质可以很好的成为一个猪尿中盐酸克伦特罗量值 溯源的载体，并且能广泛的应用到猪尿中盐酸克伦特罗检测过程的方法验证与质 量控制等方面。 关键词：盐酸克伦特罗；克伦特罗；标准物质 I ABSTRACT Clenbuterol hydrochloride, commonly known as lean, is a synthetic beta-adrenergic receptor agonist, which is used for feed additive owing to promoting animal growth, improving feed conversion efficiency and meat quality remarkably. As a series food poisoning issues with lean consumption of animal source was exposed, many countries in the world, including China, have forbidden clenbuterol to be used in the aquaculture industry legally. However, due to some reasons including the drive by economic interests, difficult detection, and insufficient understanding of the dangers to people, illegal abuse still occurs widely in many countries. At present, the forcible detection of ante-mortem urine of live specimens is very significant and effective means for the safety supervision of animal origin foods, so clenbuterol residue testing has become an obligatory item for the pigs before slaughter. The preparation of clenbuterol standard material in lyophilized powder of pig urine and evaluation are investigated in this paper. A set with five concentrations levels of clenbute