Th2试剂盒.pdfVIP

Human Th2 Cell Multi-Color Flow Cytometry Kit Catalog Number: FMC011 Size: 25 Tests Product Description This kit contains four conjugated antibodies (and corresponding isotype controls) that can be used for single-step staining of human Th2 cells (1 - 5): • IL-4 R-PE (Clone 25463; mouse IgG ) 2A • STAT6-APC (Clone 253906; mouse IgG2A) • IL-4-CFS (Clone 3007; mouse IgG1) • CD3-PerCP (Clone UCHT1; mouse IgG1) The kit also contains Fixation/Permeabilization Buffer (30 mL), which contains 1% formaldehyde, saponin, and 0.05% sodium azide and Permeabilization/Wash Buffer (60 mL), which contains saponin and 0.05% sodium azide. Intended Use This product is designed for the flow cytometric analysis of human Th2 cells using four fluorochrome-conjugated antibodies. Storage Store at 2 - 8° C in the dark. Use within 6 months of receipt. Precautions • Formaldehyde is a suspected carcinogen. Avoid contact with skin, eyes, and mucous membranes, and avoid inhaling fumes. In case of contact, wash immediately with water and seek medical advice. • Sodium azide may react with lead and copper plumbing to form explosive metallic azides. Flush with large volumes of water during disposal. Intracellular Staining Protocol with Simultaneous Fixation/Permeabilization 1. Harvest cells of interest and wash twice in PBS or Hanks’ Balanced Salt Solution (HBSS). 2. Approximately 5 x 105 washed cells should be resuspended in 0.5 mL of Fixation/Permeabilization Buffer and incubated at 2 - 8° C for 30 minutes. Cells should be vortexed intermittently in orde