液相色谱检测器介绍-实验室.pptVIP

*/32 　LC上广泛使用的各种检测器与NQAD的比较 对于没有紫外吸收的化合物，通常使用示差折光检测器（RI）来进行检测。RI的优点是不需要优化条件就可以直接进行测定，但却不能进行梯度分析，灵敏度也比较低。使用质谱(MS)可以解决这些问题，并且能够获得更多的信息，具有更高的选择性，但成本也颇高。除了以上两种方法，对于没有紫外吸收的物质，也可以使用蒸发光散射检测器（ELSD）来进行检测。而NQAD 与ELSD非常相似，但却具有更高的灵敏度。 各种检测器的特长详见下表。 对无紫外吸收物质的适用性 灵敏度 梯度分析 检测选择性 经济性 UV 不适用 ★★★★ ★★★★★ ★★★ ★★★★ MS ★★★★★ ★★★★★ ★★★★★ ★★★★★ ★ RI ★★★★★ ★★ 不可 无*注 ★★★★ ELSD ★★★★★ ★★★ ★★★★★ 无*注 ★★★ NQAD ★★★★★ ★★★★ ★★★★★ 无*注 ★★★ */32 The ENDThank You! A prefect detector design would provide all of the listed features. Unfortunately, no such detector exists and any detector design is forced to make compromises. As we will see throughout the presentation, knowledge of these compromises and the effects of the obtained results allow users to use detectors for their maximum benefit. Sensitivity is one of the most widely cited detector requirements. Generally speaking, most users demand detector that are highly sensitive to their compounds of interest. Many users describe sensitivity simply as the net response (or signal) of the peak (this may be height count, absorbance units, millivolts, intensity, and others). The true measure of sensitivity is better described as a ratio of the signal intensity divided by the intensity of the baseline noise. The diagram show the generic layout of a photo diode array detector. All of the energy from a broad spectrum lamp (or lamps) usually covering both the ultarviolet and visible wavelength range is passed through a flow cell. The light is then split into its component wavelenghts on a grating. A series of diodes measures the light intensity over the entire wavelenght range. The result is two types of data, a traditional chromatogram (time vs absorbance) at any wavelentgh in the operating range, and a spectrum (wavelength vs absorbace) at any given time point. Data collected from a PDA detector is often refered to as 3 dimensional data. This slide show a small sampling of the compound classes that are often analyzed using FL detectors. Remembe