envision(国外英语资料).doc

envision EnVision is a two step immunohistochemical technique introduced by DAKO Corporation, also known as ELPS. EnVision is generally compared to SP and ABC in color rendering: 1, to be sensitive to many, positioning better: EnVision system is the two resistance and enzyme linked into a polymer (EnVision), directly amplifying the signal 40-50 times, In combination with an already combined resistance reaction, the final chromogenic agent displays color. This method can increase the anti dilution rate by 1~2 times, especially for the weak antigen expression tissues. Some people say that SP is 1-2 times more sensitive than EnVision, but SP is not appropriate for tissues that are high in Pro biotin. 2 less nonspecific background: EnVision is a polymer that connects two resistance and enzymes, and does not contain this polymer in tissue, thus avoiding the interference of biotin in the tissue. In the tissue rich in biotin, it is better to use EnVision method. In addition to the rich tissues and necrotic tissues of blood cells, it is possible to omit the steps of endogenous peroxide closure. 3, save time: the staining procedure is two steps, and the second antibody is shorter when incubated. EnVision program: 1) dewaxing and hydrated tissue sections. 2) pretreatment of tissue slices 3) rinse distilled water and place it in TBS. (4) blocking endogenous peroxidase (used only for EnVision TM) 5) rinse the distilled water and place it in TBS for 10 minutes 6) the incubation lasted 10-30 minutes 7) rinse TBS for 10 minutes 8) EnVisionTM incubated for 10-30 minutes 9) rinse TBS for 10 minutes 10) the source substrate solution was incubated for 10 minutes 11) rinse with distilled water 12) staining and mounting -------------------------------------------------------------------------------- Immunohistochemistry (Elivison two step method) (1) the paraffin sections were placed in the oven at 67 DEG C for 2 hours, then dewaxing to water, and rinsed with pH7.4 PBS for three times,