陈润生——大数据与精准医学.ppt

Procedure The protein-protein interaction network: before and after spectral analysis The topological structure in protein-protein interaction network In clique, proteins connect quite tightly, almost interacting with each other. However, in each bipartite, proteins were divided into two parts, proteins seldom connect in same parts but connect tightly with proteins in counter part. A Clique b Bipartite The percentage of function classes in every clique Maayan A, Blitzer RD, Iyengar R Toward predictive models of mammalian cells ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE 34: 319-349 2005 Papin JA, Hunter T, Palsson BO, et al. Reconstruction of cellular signalling networks and analysis of their properties NATURE REVIEWS MOLECULAR CELL BIOLOGY 6 (2): 99-111 FEB 2005 Xia Y, Yu HY, Jansen R, et al. Analyzing cellular biochemistry in terms of molecular networks ANNUAL REVIEW OF BIOCHEMISTRY 73: 1051-1087 2004 近期的代表性文章 * * Thank you! * 1、鉴定是什么蛋白质？明确他们与疾病的关系，建立对应的疾病检测方法。 2、这些蛋白质的结构和功能是什么？哪些可以产业化开发应用？ 3、这些蛋白质的细胞定位？采用什么手段和设备可高分辨的看到他们？ 4、建立细胞平台，研究细胞水平上蛋白质的功能，并建立蛋白缺陷的特异干细胞筛选药物平台。 5、建立动物模型，研究这些蛋白质在活体中的定位和功能，并开发能特异识别病灶的分子显影剂用于诊断肿瘤等重大疾病。 6、建立中药药物筛选和评估体系，并开发重大疾病的中药药方。 7、针对没有理想动物模型的疾病，将基础与临床紧密结合，开发适合不同临床需求的诊断试剂，疫苗和药物。 上述汇报的这些大成果，大平台和大产业，依靠目前研究所的体量，体制，机制和支持是无法实现的。 miRNA-induced transcriptional gene silencingPromotor asscoiated RNA miR-10a inhibits hoxd4 transcription Transfection studies Hoxd4 expression is inhibited by miR-10a Anti-miR-10a increases hoxd4 expression Inhibition not mediated by the 3’UTR Nuclear run-on assay confirms transcriptional inhibition Transcriptional activity in the promoter region of a target gene is required for siRNA-induced transcriptional silencing PNAS 2007 miRNA不仅具有负调控作用，也可以激活基因的表达。Vasudevan 实验室发现, 在细胞周期过程中, miRNA效应在抑制作用和活化作用间摆动. 在静态细胞中(G0期), miRNA活化翻译和上调基因表达, 而在其他细胞循环/增殖期则继续发挥抑制作用. miRNA激活作用与富含腺嘌呤/尿嘧啶元件(adenylate/uridylate-rich?elements, AREs)相关. ARE是miRNA活化翻译的信号, 在miR