PCR_DGGE方法解析大豆根际不同距离土壤细菌群落多样性特征.pdfVIP

试验研究2010.6 PCR- DGGE 方法解析大豆根际 不同距离的土壤细菌群落多样性特征 1 2 1 3 3 于洪涛 申忠宝 沈德茹 金 剑 王光华 （ 黑龙江省农业科学院绥化分院 ； 黑龙江省农业科学院 哈尔滨 ； 1. 152052 2. 150086 3. 中国科学院东北地理与农业生态所 哈尔滨150081） 摘要：应用根箱法及PCR-DGGE 技术对两个大豆基因型的苗期根际不同距离的细菌群落多样性 进行研究。 结果表明，在距根面0~2mm 的范围内，细菌群落丰富度和Shannon 指数相对较高，根际 细菌的多样性优于非根际（10mm），主成分分析和聚类分析证明根际与非根际土壤的细菌群落多 样性特征存在差异。 不同基因型间苗期根际细菌群落多样性未发现显著差异。 关键词：大豆；根际；根箱； ；细菌多样性 PCR-DGGE Inspection of soil bacterial diversity along soybean rhizosphere with different distances by PCR-DGGE method (1. Heilongjiang Academy of Agricultural Sciences,Siuhua Branch, Siuhua, 152052 ； 2. Heilongjiang Academy of Agricultural Sciences, Harbin, 150086; ， 3. Northeast Institute of Geography and Agro-ecology, Chinese Academy of Science Harbin 150040 ) Abstract ： Using rhizobox method and PCR-DGGE technique, the bacterial diversity across rhizosphere in two soybean genotypes were investigated. The results showed that the richness of soil bacterial com- munity and Shannon index were relatively high at 0~2 mm of rhizosphere, indicating that the bacterial diversity in rhizosphere was better than that in bulk soil (10 mm). Principle component and cluster dendrogram analysis also confirmed the variation of bacterial diversity between rhizosphere and bulk soil. There was no difference on the bacterial diversity in rhizospheres of soybean genotypes at seedling stage. Key words ： Soybean; Rhizosphere; Rhizobox; PCR-DGGE; Bacterial diversity 根际是植物根系与土壤之间的微界面， 是生物