大孔树脂纯化管花肉苁蓉毛蕊花糖苷的工艺研究_顾云.pdf

工艺 技术 Vol. 36 , No . 04 , 2015 大孔树脂纯化管花肉苁蓉毛蕊花糖苷的 工艺研究 顾 云，孙虹霞，梁 昱，刘 昕* 中山大学生命科学大学院，广东广州510275） （ 摘 要：本研究旨在探索大孔树脂纯化管花肉苁蓉毛蕊花糖苷的方法。通过静态实验筛选纯化毛蕊花糖苷的最佳树 脂类型，并从洗脱剂浓度、洗脱时间、洗脱速度三个方面进行优化以得到该树脂动态洗脱毛蕊花糖苷的最佳方法。结 果显示：HPD300对毛蕊花糖苷的吸附和解吸能力最强，其纯化毛蕊花糖苷的最优条件为：吸附平衡后经超纯水洗脱 30min，之后依次用20%和50%的乙醇洗脱75min和60min，流速为10mL/min。纯化后，毛蕊花糖苷的含量由5.26%提高 至68.20%，回收率为68.90%。研究结果表明，采用大孔树脂HPD300纯化管花肉苁蓉毛蕊花糖苷，成本低，回收率和纯 度较高，适用于工业化生产。 关键词：管花肉苁蓉，毛蕊花糖苷，大孔树脂，纯化 Study on purifying acteoside from Cistanche tubulosa by macroporous resin * ， ， ， GU Yun SUN Hong-xia LIANG Yu LIU Xin （Grand School of Life Science ，Sun Yat-sen University ，Guangzhou 510275，China） ： Abstract The purpose of this paper was to explore method for purifying acteoside from Cistanche tubulosa by using macroporous resin. The most suitable resin was decided by static tests and then three parameters ， including eluent concentration eluting time and eluting rate were optimized for developing dynamic eluting conditions. Results showed that resin HPD300 had the best absorption and desorption capabilities. The content of acteoside increased from 5.26% to 68.20% with recovery of 68.90% under the optimized dynamic eluting ——— ， conditions eluting with ultrapure water for 30min after saturation adsorption then with 20% and 50% ethanol for respectively 75min and 60min with flow rate 10mL/min. The metho