稀土氧化物在氧化还原蛋白质直接电化学中的应用.pdfVIP

Abstract Protein and enzyme are among the most widely-used biofunctional reagents for immobilization in the field of electrochemical biosensor. They are involved in metabolism and other important physiological processes all of which contain electron transfer. The adoption of electrochemistry method will be helpful for the investigation of the electron-transfer processes of protein and enzyme. Moreover, the investigation of the direct electrochemistry of protein can help us comprehend the dynamic and thermodynamic properties of protein, establish a solid foundation to explore the biological oxidation and reduction processes, and is vitally significant for the theoretical and applicational research of new-generation biosensors, biological fuel batteries and so on. The present research takes the rare earth oxide materials (ceria and thulium) as the biological carrier of hemoglobin (Hb) and glucose oxidase (GOD) which are the biological molecules to be used to prepare a series of enzyme electrodes. It mainly discusses the application of rare earth oxides in the direct electron transfer and biological catalytic of Hb and GOD by electrochemical methods. The results show that: rare earth oxide materials can promote the electron transfer of Hb and GOD and achieve their bio-electrocatalysis. Firstly, CeO2 shows an excellent biocompatibility and provides a friendly microenvironment for the electron transfer between Hb (or GOD) and electrode. Hb immobilized on Nafion-CeO2 film retains its native secondary structure, achieves direct electron transfer with the apparent heterogeneous electron transfer rate con