高分子遗传聚合物.ppt

Catalysts from synthetic genetic polymers 从遗传聚合物合成催化剂 ABSTRACT The emergence of catalysis in early genetic polymers such as RNA is considered a key transition in the origin of life, pre-dating the appearance of protein enzymes. DNA also demonstrates the capacity to fold into three- dimensional structures and form catalysts in vitro 在早期的遗传聚合物如RNA被认为是在看待人生的起源转型的关键催化的出现，这可以追溯到蛋白质酶的外观。DNA还演示了折叠成三维结构和体外形成催化剂的能力。 However, to what degree these natural chemical scaffolds for folding or the evolution of catalysis is not known. The ability of synthetic genetic polymers (XNAs) with alternative backbone chemistries not found in nature to fold into defined structures and bind ligands raises the possibility that these too might be capable of forming catalysts (XNAzymes) 然而，到什么程度，这些天然的生物大分子，包括功能特权的化学支架折叠或催化的发展尚不清楚。聚合物的合成基因的能力（XNAs）在自然界中尚未发现能替代化学骨干折叠到定义的结构和配体结合提高的可能性,这些也可以形成催化剂(XNAzymes) Here we report the discovery of such XNAzymes, elaborated in four different chemistries (arabino nucleic acids, ANA; 2′-fluoroarabino nucleic acids, FANA; hexitol nucleic acids, HNA; and cyclohexene nucleic acids, CeNA) directly from random XNA oligomer pools, exhibiting in trans RNA endonuclease and ligase activities. 我们在这里报告xnazymes等的发现，阐述了在四种不同的化学物质（葡萄糖核酸，抗体；2′- fluoroarabino核酸，FANA；己糖醇核酸，海航；环己烯的核酸，塞纳）直接从随机XNA低聚物池，表现出反式RNA核酸内切酶和连接酶的活动式。 We also describe an XNA–XNA ligase metalloenzyme in the FANA framework, establishing catalysis in an entirely synthetic system and enabling the synthesis of FANA oligomers and an active RNA endonuclease FANAzyme from its constituent parts. 我们也描述了一个在xna–xna连接酶的酶催化在FANA框架，并且在一个完全建立催化合成系统，使FANA低聚体和一个活跃的RNA核酸内切酶FFANAzyme从其组成部分合成。 These results extend catalysis beyond biopolymers and establish technologies for the discovery of catalysts in a wide range of polymer scaffolds not found in nature. 这些结果扩展催化以外的生物聚合物为催化剂的发现和建立技术广泛的聚合物支架在自然界中尚未发现。 Evolution of catalysis independent of any natural polymer has implications fo