原发性肝癌p14ARF基因CpG岛甲基化研究.pdfVIP

· 848· 生11 第22卷第11期 Chin J Gen Surg．November 2007，V01．22．N0．11 ． 论著 原发性肝癌pl4ARF基因 CpG岛甲基化研究 阮新贤 王少平 陈孝平 任培土 【摘要】 目的 探讨pl4ARF基因启动子区CpG岛甲基化与原发性肝细胞肝癌发生、发展的关 系。方法 采用逆转录一聚合酶链式反应(RT—PCR)检测42例原发性肝癌组织及癌旁组织中 pl4ARFmRNA的表达，同时用甲基化特异PCR(methylation—specific PCR，MSP)方法分析pl4ARF基因 启动子区域甲基化状况。 结果 42例肝癌组织有7例呈p14 mRNA的表达，表达率17％。癌旁正 常组织均有p14表达。pl4mRNA在原发性肝癌组织中明显表达缺失(x =56．62，P0．05)；42例原 发性肝癌组织中，有19例检测到p14基因启动子的甲基化，甲基化发生率45％。相应癌旁组织未发 现甲基化。pl4ARF基因在原发性肝癌呈现明显高甲基化()(2=22．04，P0．05)。在pl4mRNA表达 的7例肝癌组织中没有检测到p14基因启动子的甲基化，而在pl4mRNA表达缺失的35例肝癌组织 中，19例p14基因启动子呈现甲基化。肝癌组织中pl4ARF基因启动子甲基化与pl4mRNA表达缺失 明显相关( ：4．66，P0．05)。 结论 在原发性肝癌p14 mRNA明显表达缺失，原发性肝癌 pl4ARF基因启动子频繁甲基化可能是pl4ARF失活的原因之一。 【关键词】癌，肝细胞；基因表达；pl4ARF； 甲基化 5 CpG methylation of pl4ARF gene in hepatocellular carcinoma RUAN Xin—xian ． ，vG Shao— ping，CHEN Xiao-ping，REN —tu．‘Department of General sⅡ ，Shaoxing Pe~le S Hospital， Shaoxing 312O00．China 【Abstract】 0bjeetive To explore the relationship between the methylation at pl4ARF locus and the progression of hepatocellular carcinoma(HCC)． Methods Reverse—transcription polymerase chain reaction(RT．PCR)assay was used to examine the expression of pl4ARF mRNA in 42 HCC samples and corresponding noncancerous tissues． Methylation—specific PCR(MSP)Was performed to detect the methylation at pl4ARF promoter re on in those HCC tissues and matched adjacent noncancerous liver tissues． Results RT．PCR assay revealed pl4ARF positive mRNA expression in 7 of 42(17％)in HCC tissues and all of corresponding noncancerous tissues．Compared to corresponding normal tissues，pl4ARF mRNA level Was significantly decreased in HCCs tissues( =56．62，P0．05)．Aberrant promoter methylation of the p14 gene was detected by MSP technique in 19 of 42(45％)HCC tissues，and there Was no methylation found in adiacent noncancerous tissues(Y =22．04．P0．05) In 19 out of