乳状液的组成及油滴粒径对β-胡萝卜素生物接近度的影响.pdfVIP

22 2015， 协，1.36， No.09 ※基础研究 国目融事 乳状液的组成及油滴粒径对p-胡萝卡素 生物接近度的影响 张金边，樊金玲*，杨睿，朱文学，孙晓菲 (河南科技大学食品与生物工程学院，河南洛阳 471∞3) 摘 要:目的:研究乳状液中ß-胡萝卡素的胶束化过程以及油滴粒径、油相含量和介胡萝卡素质量分数对乳状液 中β胡萝卡素生物接近度的影响规律及可能机制。方法z 采用静态体外消化法，以肢束化率为指标评估β胡萝卡 素的生物接近度。结果:肠消化阶段的前30 min是乳状液中ß-胡萝卡素胶束化过程的重要阶段:在一定范围内， P胡萝卡素的胶束化率(3.239毛-10.87%) 与乳状液油滴粒径 (0.44-4.28 f.1IIl)呈线性负相关:油相含量1%-20% 范围内， ß-胡萝卡素的胶束化率随泊相含量增加呈 S 型增长趋势:β胡萝卡素的胶束化率随其质量分数的增加 (2-30mgll∞g) 先上升后降低，相应地胶束中ß-胡萝卡素的绝对质量先上升而后进入平台期。 关键词:乳状液:油滴粒径; ß-胡萝卡素:高压均质:体外消化法:生物接近度 Effect of Emulsion Composition and Oil Droplet Size on ß-Carotene Bioaccessibility ZHANG Jindi , FAN Jinling , YANG Rui , ZHU Wenx时， SUN Xiaofei * (College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471∞3， China) Abstract: Purpose: To investigate the process of ß-carotene micellarization and evaluate the effect of oil droplet size, oil phase content and ß-carotene concentration on the bioavailability of ß-carotene incorporated in emulsions as well as to elucidate the possible mechanisms. Methods:τhe ß-carotene bioaccessibility was assessed by in vitro digestion model. The fraction of ß-carotene transferred from the emulsion to a micellar phase was investigated. Results: Wi也in the first 30 min of duodenal digestion, the majority of lipid hydrolysis and ß-carotene micellarization occurred. There was a negative correlation between the ratio of ß-carotene incorporated into micelles and oil droplet size in 由e range from 0.44 to 4.28 阳n. When the oil phase content increased from 1 % to 20% , the bioaccessibility of ß-carotene in the micellar p