免疫组化操作步骤(国外英语资料).doc

免疫组化操作步骤(国外英语资料) The documents I have carefully collated are from the web I only collect the collation If there are any mistakes Still ask oneself to check! Steps of immunisation (1) instruments and equipment 18cm stainless steel pressure cooker or electric stove or microwave oven. 2. Water bath pot (2) PBS buffer (ph7.2-7.4) : NaC137mmol/L KCl 2.7 mmol/L, Na2HPO4, 4.3 mmol/L, KH2PO4 1.4 mmol/L. 2.0.01 molar of sodium citrate (CB, ph6.0, 1000ml) : citric acid trisodium 3g, citric acid 0.4 g 3.0.5 mol/L EDTA buffer (ph 8.0) : 700ml of water dissolved in water. 2H2O, set 10mmol/L NaOH to ph8.0 and add water to 1000ml. 1 molar of TBS buffer (ph8.0) : dissolve 121gTris in 800 ml water Use 1N HCl to ph8.0, plus water 1000ml Enzyme digestive fluid: a. 0.1% trypsin: use 0.1% CaCl 12 (ph7.8) B. 0.4% gastric protease fluid: use 0.1 N HCl 3% methanol - H2O2 solution: 30% H2O2 and 80% methanol solution Wind mounted agent: a. Glycerin and 0.5 mmol/L carbonate buffer (ph9.0-9.5) with a mixture of b oil and TBS (PBS) TBS/PBS ph9.0-9.5 for fluorofiberscope; Ph7.0-7.4 is suitable for optical fiber specimens (3) procedures of operation 1, dewaxing and hydration: before dewaxing tissue chip should be placed in room temperature 60 minutes or 60 ℃ bake for 20 minutes in the constant temperature box A tissue chip is immersed in dimethylbenzene for 10 minutes, and after replacing xylene, soak for 10 minutes Soak for five minutes without water C 95% ethanol for five minutes D 75% ethanol for five minutes Antigen repair: used in formalin fixation of paraffin embedding tissue chips: A antigen heat repair A high pressure heat repair is added to the boiling water to add EDTA (ph8.0) or 0.01 m citric acid sodium buffer solution (ph6.0). Cover stainless steel pot cover But it cant lock Put the glass on a metal stain Slow pressure The slide is soaked in the buffer for five minutes Then lock the lid Small valves will rise Remove the heat source in 10 minutes Put in the cold water Open the lid whe