合成纳米铝佐剂及其对乙型肝炎_狂犬病毒辅佐效应的研究_何萍.pdfVIP

DOI :10.13431/j.cnki .immunol.j ·90 · 　 　22 　 1　2006 1 　　IMMUNOLOGICAL JOURNAL Vol.22 No.1 Jan.2006 [] 1000-8861(2006)01-0090-04 、 a b ＊ b b b b 何　萍, 吕凤林 , 陈　月 , 左国伟, 李元朝, 何凤慈 (第三军医大学 a.西南医院胸心外科, 重庆 400038;b.大坪医院野 外科研究所第一研究室, 重庆400042) [　] 　　, 。 　25 ℃, 。 , Balb c , IgG 。　(TEM)(D C), 72.62 nm, Al(OH)。 3 HBsAg, Balb c 1 2 (P 0.01;P 0.05); , IgG , 7 , (P 0.05)。 　HBsAg Rabies , Balb c 。 [] 　;;;; [ ] 　R392.11　　　　[] 　A Synthesis of nanoparticulate alum adjuvant and its application to the HBsAg and rabies virus HE Ping, LV Feng-lin, CHEN Yue, ZUO Guo-wei, LI Yuan-chao, HE Feng-ci (Department of Cardiothoracic S urgery , Southwest Hosp ital, Third Military Medical University , Chongqing 400042, China ) [Abstract] 　Objective 　To study the humoral immunity changes induced by HBsAg and rabies virus absorbed onto domestic nanoparticulate alum adjuvant.Methods　Nanoparticulate alum adjuvant had been synthesized by the microemulsion at 25 ℃.The specific an- tibody titers of IgG in Guinea pigs and Balb c mice were assayed after hypodermic injection of nanoparticulate alum adjuvant, and then com- pared with those of normal alum adjuvant.Results　By the analyses of transmission electron microscopy (TEM)and differential scanning calo- rimetery (D C), the resultant particle was characterized to be the Al(OH)crystal, which was a spherical structure of 70 nm in diameter. e- 3 rum anti-HBsAg IgG titers of nanoparticulate alum adjuvant group were higher than those of bulk alum adjuvant group in the first two weeks after immunization (P 0.01;P 0.05);The anit-rabies virus IgG titers of nanoparticulate alum adjuvant group was higher than those of con- trol group (P 0.05), and the antibody presented positive at 7 d postimmunization.Conclusion　With assist of nanop