大鼠帕金森病发病过程中黑质细胞凋亡及其相关基因表达.pdfVIP

曹 非等 大鼠帕金森病发病过程中黑质细胞凋亡及其相关基因表达 第24期 · 2369· 大鼠帕金森病发病过程中黑质细胞凋亡及其相关基因表达 曹 非 骆 芳 段国安 (华中科技大学同济医学院附属协和医院神经内科，湖北 武汉 430022) [摘 要】 目的 观察帕金森病(Parkinson disease，PD)发病过程中黑质细胞凋亡变化规律，观察相关黑质细胞凋亡基因p53、Bax、热休克蛋白 (HSP)的表达。方法 通过脑立体定位注射6一羟基多巴胺(6-0HDA)的方法建立大鼠PD模型，采用TUNEL方法、免疫组织化学、尼氏染色等方法， 选择术后1、7、14、21 d为研究时点，观察大鼠PD模型形成过程中尼氏细胞、黑质细胞凋亡的数量及相关基因变化情况，并检测黑质细胞p53、Bax、 HSP表达情况。结果 用TUNEL法发现黑质细胞存在细胞凋亡，与对照组存在显著性差异(P0．05)，各时点黑质细胞凋亡数逐渐增加，尼氏细胞 则逐渐减少，随时间增加而升高，p53和HSP则在1 d为最高，其后很快下降，但都高于对照组(P0．05)，Bax蛋白表达逐渐减少。结论 PD发病过 程中黑质细胞凋亡参与其中，呈现一定变化规律，并受到p53、Bax和HSP的影响。 [关键词]6一羟基多巴胺；帕金森病(PD)；细胞凋亡；黑质 [中图分类号] R742．5 [文献标识码] A [文章编号] 1005-9202(2007)24-2369-03 Research oil the substantia nigral apoptosis during the episode of Parkinson disease and protein expression of the related gene CAO Fei．LUO Fang．DUAN Guo-An． Department of Neurology，Xiehe Hospital，Tongji Medical College，Huazhong Scientific and Technologic University， Wuhan 430022，Hubei，China 【Abstract】 Objective To obser~e the rule of apoptosis in substantia nigra and protein expression of relative gene p53，Bax， HSP．Methods TUNEL method，Nissl staining and immunohistochemical techniques were used to observe the number of nigral apoptosis and Nissl neuron in the course of the formation of Parkinson disease models in rats(PD group)on 1，7，14，21 d after 6-OHDA injected stereotaxi- cally to fight side of mesencephic ventral tegmental area and substantia nigra pars compacta of rats．At the same time，p53，Bax，HSP protein expression were measured．Results The number of substantia nigral apoptosis in the PD group Was much more than that of the control group (P0．05)，and Was maximum on 1 d and lowest on 21 d．p53，HSP attained the peak value promptly on 1 d，and then decreased rapidly．Bax protein decreased gradually．Conclusions Substantia nigral apoptosis could be involved in PD with certain change regularity and changed， which is regulated by p53，Bax，HSP． 【Key wor