鲫鱼汤对阿霉素肾病大鼠Th17细胞相关炎性因子表达影响.docVIP

鲫鱼汤对阿霉素肾病大鼠Th17细胞相关炎性因子表达的影响* 张勇1，张蓓2*，邢广群3，宁华英4，王金香1，郭云良4，沈卫4 （1.青岛大学医学院，2.青岛大学医学院免疫学教研室, 3.青岛大学医学院附属医院，4.青岛大学医学院中西医结合中心 山东 青岛266003） 【摘要 目的 研究鲫鱼汤对阿霉素肾病大鼠IL-17、IL-23、IL-1β和CXCR2等表达的影响，探讨其治疗作用和机制。方法 成年健康 Wistar雄性大鼠40只，随机分为鲫鱼汤组、厄贝沙坦组、肾病组、空白对照组，除空白对照组，其他三组给予尾静脉注射6.2mg/kg体重阿霉素建立肾病模型，空白组注射等容量生理盐水。模型成功后，给予鲫鱼汤灌胃干预9周，每周检测12h尿蛋白定量，检测终末血生化指标变化，光镜下观察大鼠肾脏病理改变，免疫组化检测IL-17、IL-23、IL-1β和CXCR2在肾脏的表达，ELISA法检测IL-17、IL-23和IL-1β在血液中的含量。结果 鲫鱼汤组大鼠血白蛋白水平高于肾病组(P=0.03)低于空白组(P=0.01)；鲫鱼汤组大鼠肾组织IL-17、IL-23、CXCR2较肾病组表达减少(P=0.01，P=0.01，P=0.02)，较空白组表达增多(P=0.04，P=0.007，P=0.05)。结论 Th17细胞相关炎性因子增多是阿霉素肾病大鼠的发病机制之一，鲫鱼汤可通过升高血白蛋白，降低IL-17、IL-23、CXCR2表达而发挥肾脏保护作用。 【关键词The positive effect of Crucian carp decoction on the expression of Th17 cells inflammatory factors in rats with nephrosis induced by adriamycin ZHANG Yong1,ZHANG Bei2,XING Guang-qun3,NING Hua-ying4,WANG Jin-xiang1,GUO Yun-liang4, SHEN Wei4 (1.Qingdao University Medical College,2. Department of Immunology, Qingdao University Medical College, 3.The Affiliated Hospital of Medical College Qingdao University,4.Institute of Integrative Medicine, Qingdao 266003,Shandong, China.) 【Abtract】 Observe the therapeutic effect of Crucian carp decoction on rats with nephrosis induced by adriamycin, and explore the mechanism. Methods Fourty male Wistar rats were randomly divided into Crucian carp decoction group、Irbesartan group、Nephrosis group，and Control group. Nephrosis of rats were induced by a single tail intravenous injection of adriamycin 6.2mg/kg body weight.The rats were administered once oral gavage of Crucian carp decoction group for 9 weeks, urinary protein was checked each week.All rats were killed at week 9.Histological changes were observed by light microscopy and blood biochemical indices were detected. IL-17、IL-23、IL-1β、CXCR2IL-17、IL-23、IL-1β Rats in Crucian carp decoction group had a higher level of albumin compared with Nephrosis group (P=0.03),a lower level compared with Control group(P=0.01) and the expression of IL-17、IL-23、CXCR2 in ki