近红外光谱技术定量分析玉米杂交种纯度.pdf

第28 卷 增刊2 农 业 工 程 学 报 Vol.28 Supp.2 2012 年 10 月 Transactions of the Chinese Society of Agricultural Engineering Oct. 2012 259 Quantitative analysis of seed purity for maize using near infrared spectroscopy 1 1 1 2 3 1※ Wang Qing , Xue Weiqing , Ma Hanxu , Li Junhui , Sun Baoqi , Sun Qun (1. Department of Plant Genetics and Breeding, College of Agriculture and Biotechnology, China Agricultural University/Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193, China; 2. College of Information and Electrical Engineering, China Agricultural University, Beijing 100193, China; 3. Beijing Forestry Academy Science, Beijing 100097, China) Abstract: A quantitative identification model for testing the purity of Xianyu335 hybrid maize seed was built by near infrared reflectance spectroscopy (NIRS) with quantitative partial least squares (QPLS). By grinding and mixing maize hybrid seeds of different years and sources with their female parent seeds, 123 samples were obtained with a 0.5% gradient and purity within the range of 80%-100% (three replicates of every year and every source in each gradient) and the spectra of the samples were collected. The results showed as following: through implementation of scatter correction pretreatment, the wave number range of 4 000-8 000 cm-1 was appropriate for modeling (calibration sets: validation set = 2 3:1); the i