工作场所空气中氯丙酮气相色谱测定方法探究.docVIP

工作场所空气中氯丙酮气相色谱测定方法探究摘要 ： ［目的］ 建立工作场所空气中氯丙酮气相色谱测定方法。 ［方法］ 硅胶吸附空气中蒸汽态氯丙酮，经丙酮溶剂解吸后气相色谱分离，并由电子捕获检测器（ECD）测定。 ［结果］ 氯丙酮的浓度在2.30～57.5 μg/ml时，相对标准偏差（RSD）为2.7%～3.6%（n = 6）；回归方程y#710; = 57 + 95 x，相关系数r 0.999 5；方法检出限为0.23 μg/ml（进样1.0 μl）；最低检出质量浓度0.12 mg/m3；平均解吸效率 98%；穿透容量（BTV） 0.69 mg（100 mg硅胶）；100 mg硅胶吸附11.5 μg氯丙酮，密闭放置7 d，下降率为3.5%；采样效率100%。 ［结论］ 本方法适宜于工作场所空气中氯丙酮的浓度测定 关键词：氯丙酮；工作场所空气；硅胶管；溶剂解吸；气相色谱 A Study on Gas Chromatographic Detection of Chloroacetone in Air of Workplace CAI Yue1，DING Jin-chun1，CAO Ming1，WANG Dong-jiu1，YANG Sheng-qin1，XU Yi-sheng2（1.Shanghai Minhang District Center for Disease Control and Prevention，Shanghai 201100，China；2. Shanghai Municipal Centerfor Disease Control and Prevention，Shanghai 200336，China） Abstract：［ Objective］ To establish a method of GC detection of chloroacetone in the air of workplace. ［Methods］ The chloroacetone vapor in the air is absorbed by silica gel，desorbed by acetone solvent，separated through gas chromatography，and detected by electronic capture detector（ECD）. ［Results］ When the concentration of chloroacetone is in the range of 2.30 ～57.5 μg/ml，the relative standard deviations（RSD）is between 2.7%～3.6%（n=6）；the regression equation is y#710; = 57 + 95 x，with a correlation coefficient r0.999 5. The detection limit of this method is 0.23 μg/ml（injection1.0 μl），the lowest concentration detected is 0.12 mg/m3，the average desorption efficiency 98%，and the breakthrough volume（BTV） 0.69 mg（100 mg silica gel）. With 11.5 μg chloroacetone absorbed by 100 mg silica gel and stabilized airtightly for 7 days，the drop rate of chloroacetone content is 3.5%；sampling efficiency reaches 100%. ［Conclusion］ This method is adaptable to detection of chloroacetone concentration in the air of workplace. Key Words：chloroacetone；air in workplace；silica gel tube；solvent desorption；gas chromatography 氯丙酮是一种无色液体，有极强的刺激性气味和催泪性。相对分子质量92.53，密度1.123 g/cm3（25/4 ℃），沸点119 ℃［1］。 常作为燃料、农药、医药生产的原料。质量浓度在18 mg/m3时致流泪，在100 mg/m3时不能忍受。急性毒性：大鼠经口LD50 为100 mg/kg；兔