藻胆蛋白质的提取纯化与生物活性研究进展 - 中国科学院.pdf

生物技术通报 ·综述与专论 · B IO TECHN OLO G Y　BULL E T IN 20 10 年第 1期 藻胆蛋白质的提取纯化与生物活性研究进展 张唐伟1, 2 　李天才1 ( 1中国科学院西北高原生物研究所 ,西宁 810008; 2 中国科学院研究生院 ,北京 100049) 　　摘 　要 : 　藻胆蛋白的提取原料主要来自于红藻和蓝藻 ;细胞破碎的方法主要有反复冻融法 、化学试剂处理法等 5 种方 法 ;提取的方法主要有盐析法等 3种 ;分离纯化的方法主要有层析法等 3种 ;藻胆蛋白其生物活性主要表现在 :抗肿瘤活性 、抗 病毒活性 、抗氧化和消炎活性 ,提高免疫活性 。 关键词 : 　藻胆蛋白 提取纯化 生物活性 Rev iew on Extraction Pur if ica tion and B iolog ica l Activ ity of Phycob iliprote in 1. 2 1 Zhang Tangwei L i Tiancai 1 ( N orthw est Institu te of P la teau B iology, Ch inese A cadem y of S cience, X in ing 810008; 2 Gradua te Un iversity, Ch inese A cadem y of S cience, B eij ing 100049) 　　Ab s tra c t: 　Through refer to recen t literature abou t p hycob ilip rotein, we found the u sual extracting m aterials of p hycob ilip rotein were concluded as rhodophyta and algae. There were five m ethod s to crash cell, m ain ly including rep eated freezing and thaw ing, chem i cal reagent. There had three m ethod s of extraction such as the saltingou t, and three m ethod s of sep aration and pu rification such as chro m atograp hy. The b iology activity of phycob ilip rotein m ain ly disp lays on an titumor, antiviral, oxidation and antiinflamm atory, imp rove imm un ity activity. Key w o rd s: 　Phycob ilip rotein Extraction and pu rification B io logical activity ( ) 藻胆蛋白 p hycob ilip rotein , PB P 是藻类特有的 白和别藻蓝蛋白在所有的蓝藻和红藻中都有分布 ,