MDV-1 VP22 羧基端在大肠杆菌中高效可溶性表达 - Virologica Sinica.PDF

21 2 21 2169-172 2006 3 VIROLOGICA SINICA March 2006 MDV-1 VP22 * ** 225009 Highly Soluble Expression of Carboxyl Terminus of Marek’s Disease Virus VP22 in E. coli ** CHEN Hong-junSONG Cui-pingQIN Ai-jian LIU Yue-long and JIN Wen-jie (Key Lab of Jiangsu Preventive Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China) Abstract Viral protein VP22 is indispensable for the growth and replication of Marek’s disease virus serotype 1(MDV-1). In this study, vp22 gene was amplified from and avirluent CVI988/Rispens strain. Antibodies specific to the carboxyl terminus of VP22 expressed in E. coli were generated by immunizing Balb/C mice 3 times. The antibodies reacted with the complete VP22 expressed in Chick Embryo Fibroblast (CEF) cells infected with CVI988 virus. It is interesting that the VP22 protein could be found in normal CEF cells around MDV plagues. The results suggested that VP22 may be transported among cwlls in MDV infection, which is vital to the studies on functions of VP22. Key words Marek’s disease viru s (MDV), CVI988/Rispens strain, VP22, E. coli, protein transduction VP22 I MDV-1MDV-1 CVI988/Rispens DNA VP22 C SDSVP22 C 42kDa 6 Balb/C VP22 C VP22 MDV CEF VP22 CVI988/Rispens VP22 S831.7 A 1003-5125(2006)02-0169-04 I Marek’s disease [3,4]MDV-1 VP22 HSV-1 virus serotype 1MDV -1 UL49h I Protein transduction domainPTD Herpes Simplex Virus serotype 1 Hung [5]MDV-1 VP22 16 HSV-1 UL49 VP22 HPV-16E7 DNA 27.6kDvp22 Yanagida [1]MDV-1 E7 CD8+ GA 8.4kb BamH -Eco