鹿场环境中细菌的分布及耐药性调查.pdfVIP

2017 44 3 904911 China A nim al H usbandry Vete r inary M e d ic ine doi cnki j S rDNA PCR CFU CFU mL g S rDNA NCBI B S A Distribution and Drug Resistance Investigation of Bacteria from Deer Farm Environment WANG Chao Z HANG Mengnan NIE Lin SHEN Naiw en LIU Xin ZA HNG Yi HE Jianbin YIN Ronghuan College of A nimal H usbandry and Veterinary Medicine Sheny ang A g ricultural Univ ersity Sheny ang China Abstract T he ex periment w as aimed to identify the bacterial distribution and drug resistance situ ation in deer f arm environment f orm Xif eng county Liaoning province We collected manure w ater air and f eed samples f rom deer f arm f or isolation and identification of bacteria bacterial count bacterial S rDNA PCR amplification sequencing analy sis and the pathogenic bacteria and conditional pathogenic bacteria of drug sensitivity test T he result s show ed that there w ere strains of bacteria isolated and purified enumeration of male and f emale deer s pellet f eed f odder and w ater w ere CFU and CFU m L respective g ly Description of w ater and f odder did not conf orm to the standard of the national health require ment s S rDNA gene sequences comparison in NCBI show ed that these bacteria belong