骨髓间充质干细胞对脑缺血大鼠轴突生长及AKTGSK-3βCRMP-2表达的影响.pdfVIP

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骨髓间充质干细胞对脑缺血大鼠轴突生长及AKTGSK-3βCRMP-2表达的影响.pdf

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骨髓间充质干细胞对脑缺血大鼠轴突生长及AKTGSK-3βCRMP-2表达的影响

目录英文缩略词表 ··················································3 中文摘要······················································4 英文摘要······················································6 前言························································8 第一部分大鼠骨髓间充质干细胞分离培养及鉴定引言······················································11 材料与方法················································11 结果······················································15 讨论······················································16 第二部分骨髓间充质干细胞移植对局灶性脑缺血大鼠的行为学影响引言······················································18 材料与方法················································18 结果······················································24 讨论······················································26 第三部分骨髓间充质干细胞对脑缺血大鼠轴突生长及蛋白 pAKT 、p GSK-3 β pCRMP-2 、GAP-43 表达的影响表达的影响引言······················································28 材料与方法················································28 结果······················································34 讨论······················································36 结论··························································38 论文参考文献··················································38 致谢························································48 综述························································49 综述参考文献··················································56 2 万方数据英文缩略词表 (Abbreviations and acronyms) AGE Agarose Gel Eleetro Phoresis 琼脂糖凝胶电泳 BMSCs bone marrow -derived mesenchymal stem cells 骨髓间充质干细胞 BSA Bovine