生物复混肥对土壤微生物功能多样性及土壤酶活性的影响.pdfVIP

农业环境科学学报 2012，3 1(6):1153-1159 Journal of Agro-Envimnment Science 生物复混肥对土壤微生物功能多样性及 土壤酶活性的影响 邵哥1 ，谷洁2掌，张社奇 l ，高华2，秦清军 2 ( 1 西北农林科技大学f理学院.陕西杨凌 7121∞; 2.商北农林科技大学资源环境学院.陕西杨凌 7121ω) 摘 要:在温雪盆裁条件下，采用 Biolog 微乎板技术，研究了王米施用等养分量的无机~E 有机无机复混肥、生物复混肥后土壤微生 物群落功能多样性及士填酶活性的动态变化。结果表明，生物复混肥处理的微牛，物群落乎均颜色变化率 (AWC川、微生物群落 5hannon 指数(H) 、丰富度指数(S)和 Shannon 均匀度指数(E)均为鼓高;微生物群落主成分分析表明，不同施肥处理土壤做牛物群 落碳源利用特征有一定差异，PC1 将生物复混月E 与其他处理-明显区分，牛物复混肥处理分布在 PCI 的正方向，其他处理分旬在 PC1 的负方向:起分异作用的主要碳源在糖类、竣酸类和l 氨呆酸类:士-攘舷糖梅、服酶活悻均以生物复混肥处理最高，分别为 72川mg I I g1uc值eg- (24 h)-I 和1.15 mg NH ,-N.g (3 ht10 研究表明，生物复混肥的施用比等养分量的1i机无机复混肥处理能显著提高土 壤微生物群落碳盟军利用率、微生物群落的丰富度和功能多样怜，增强上壤庶糖酶和服酶活性。 关键词:生物复混月E; 功能多样件:土壤微牛-物群落;土模酶活性 申图分类号:5144.1 文献标志码:A 文章编号: 1672-2043(2012)侃-1153-07 Effects of Bio-compound Fertilizer 00 Corn Soil Microbial Community and Enzyme Activities SHAO [j, GlJ Jie气 ZHANGShe→1il， GAO Hua, QIN Qîng-jun2 (I.College of Sciences.Northwest A F University. Yangling 7121∞. China; 2.College of Resources and Environment. Northwest A F University. Yangling 7121∞. China) Abstract: Fertîlization could înfluence 50îl ph同时hemical and biologic.al properti四 By the biolog micro-pJate technîque嘈 this study investi- .er. organic-inorganic fertilizer. inorganîc fertilizer at 由e same nutrient amount of N, P and K on gated the effects of bîo-compound fertili7 functional diversilies of 回il microbe communities and 酬1 enzyme aclivities under com pot时 experiment în greenhouse. The substrates of Biolog ECO plate were composed of six types of substances( carbohydrales. carbo巧lic acids. amino acids , polymeæ , amines and aromatic chemicals). Principal component analysis was used to show the difference utilization of substrates by 80il microbes. The results 写howed Ihat the Average Well-Color DeveIopments{A WCD), Shannon index{H) , Richness index{S) and