人血浆中红霉胺的LC-MSMS法测定.pdfVIP

• 362 • 中国医药工业杂志 Chinese Joumal of Phannaceuticals 2008 , 39 (5) 人血浆中红雹肢的LC-MS/MS法测定 郭立志l ，赵曦2 ，杨汉煌2 ，殷立新1 ，任进民1* (1.闸北医科大学第二医院，河北石家庄 050000: 2. 中奇制药技术布限公司，河北石家庄 050051) 摘要:建立了LC-MS/MS法测定人血浆中红稳肢的含壤。样品用回相望在取提取，采用C 柱，流动相为甲醇-0.5% 甲酸水 18 溶液(50: 50) ，以盼妥拉明为内标，电喷雾离子化，选择正离子多离子反应监测(MRM) ，检测离子分别为m/z 735.7• 577.5(红霉胶)和m々 282.2叶212.2( 内标)。红霉胶在5-1000ng!ml浓度范围内线性关系良好，方法回收率大于93% ，提取 回收率大于90% ，日内和日间RSD小子4.8%. 关键诩:红霉胶:液相ß谱-串联质谱:回相声在取:测定 中图分类号: TQ460.7+2; 0657.63 文献标识问:A 文章编号: 1001俑8255 (2008) 05-0362心4 Determination of Erythromycylamine in Human Plasma by LC翩MS/MS 2 J GUOLi嗣zhi\ ZHAO xe,YANG Han-严1 ， YIN Li翩xin\ REN Jin-min * (1. The Second Hospital 01Hebei 胁dical Universi秽\Sh扩iazhuang 050000; 2. Zhongqi Pharmaceutical Technology Co., Ltd. , Shijíazhuαng050051) ABSTRACT: A LC-MSIMS method was established for the determinatíon of erythromycylamine in human plasma. ηle samples were treated by solid phase extraction with average extractíon recovery was above 90 %. A C18 column was used with the mobile phase ofmethanol-O.5% formic acid(50: 50). Phentolamine was used 拙 intemal standard. ESI W制 applied and operated in positive mode. The detected íons were m.々 735.7→577.5 (erythromycylamíne) and m.々 282.2• 212.2 (phentolamine). The calibration curve was linear in the range of 5 - 1000ng/ml. The average recovery was more than 93 %.机le RSDs of inter- and intra-day were less than 4.8 %. Key Words: erythromycylamine; LC-MSIMS; solid phase extractíon; determination LC翩 MS[3-Sl 法测定血浆中1 的浓度。本研究建立 地红寄至素 (dirithromycin) 是新一代大环内醋 类抗