人血浆中齐拉西酮的LC-MSMS法测定.pdfVIP

中国医药工业杂志 Chinese Journal of Phannaceuticals 2008 , 39 (3) • 203 • 人血浆中齐拉回酬的LC-MSIMS法测定 周 练，葛庆华气式晓1辈，马丽丽 (上海医药工业研究院，药物制剂国家工程研究中心，上海 201203) 摘要z 建立了回相萃取LC-MS/MS法测定人血浆中齐拉西酬浓度，采用CJS柱，流动相为甲醇·水·甲酸(60 : 40 : 0.02 , 含5mmol/L酷酸馁， pH 5.0). 电离模式为ESI 电离，扫描类型芷离于扫描.在0.5-400ng/ml范围内线性关系良好 。叶.9999) ，最低定量限为0.5nglml. 平均凹收凉大于93%. 日内及日间RSD均小于10%. 关键诩2 齐拉西嗣:液相ßt曹·串联质谱:测íÈ 中图分类号: TQ460.T2; 0657.63 文献标识码:A 立章编号: 1001-8255 (2008) 03翩0203冶3 Detennination of Ziprasidone in Human Plasma by LC翩MSIMS ZHOU Zhen , GE Qing-hua ,ZHI Xiao气jin. MA Li-li (Shanghai lnstitute ofPharmac倒阳11ndus町\ National Pharmaceutical Engineering Research Center, Shanghai 201203) ABSTRACT: A LC-MS/MS method with solid-phase extraction was established for the determination of ziprasidone in human plasma. A C18 column was used , with the mobile phase of methanol幽water.也rmic acid (60; 40 :0.02 , containing 5mmol/L ammonium acetate. pH 5.0). The LιMSIMS system used an ESI in positive scanning. The calibration curve W制 linear in the concentration range of 0.5 帽 400ng/ml (r=0.9999). The limit of quantífication was 0.5ng/ml. The avera伊recovery was above 93 %, the RSDs of intra- and inter-day were less than 10%. Key Words: ziprasidone; LC-MSIMS; determination LC翩MS/MS系统: API3000ru!质谱仪(英国应用生物系统 齐拉西酣(ziprasidone ， 1) 是新的非典型抗精神 公司)，高放液相缸谐仪，包括LC-lOADvp泵、 SIL-HTC自动进 病药物，对精神分裂症或分裂情感性精神障碍者具 样棒、 CTO-IOAvp柱翻箱(日本岛津)。 有良好的作用，井有较好的抗焦虑作用。该药的不 甲磺酸1对照品(含1 79.71%)和内标(格列毗嚓.2. 含量 良反应少，很少会引起锥外反应，几乎没有高催乳 99.6%) 均由本院提供:甲醇、甲酸为色谱纯，酣酸服为分析 萦血症，见效迅壤。荣回 FDA B批准辉瑞公司的 甲横酸1 (商品名Gerdo