羟乙基化牛膝多糖的合成及其活性研究 - 上海有机化学研究所.pdfVIP

2003 年第 61 卷 化 学 学 报 Vol. 61 , 2003 第 10 期 , 1692～1696 ACTA CHIMICA SINICA No. 10 , 1692 ～1696 ·研究简报· 羟乙基化牛膝多糖的合成及其活性研究 张　健　　田庚元 ( 中国科学院上海有机化学研究所 　生命有机化学国家重点实验室 　上海 200032) ( ) 摘要 　以环氧乙烷为羟乙基化试剂,在碱性水溶液中 ,对牛膝多糖 Abps 进行羟乙基化 ,经过丙酮沉淀、膜分离、Sephadex ( ) G25 柱层析等分离方法得到羟乙基化牛膝多糖 AbpsHE 纯品 ,检测其理化性质 ,并通过甲基化、GCMS 分析 ,初步确证糖 链中羟乙基主要取代在葡萄糖 6 位和果糖的 1 位或 6 位上. 药理实验表明 ,羟乙基化牛膝多糖对荷Lewis 肺癌小鼠NK 活性 具有一定促进作用. 关键词 　牛膝 ,羟乙基化牛膝多糖 ,结构分析 ,抗肿瘤活性 Studies on Synthesis , Structure and Bioactivity of Hydroxyethylated Abps ZHANG, Jian 　　TIAN , GengYuan ( State Key Laboratory of Bioorganic and Natural Products Chemistry , Shanghai Institute of Organic Chemistry , Chinese Academy of Sciences , Shanghai 200032) Abstract 　Achyranthes bidentata polysaccharides (Achyranthan , Abps) was hydroxyethylated by ethylene oxide in aqueous NaOH. The crude product was purified by acetone precipitation , dialysis , and Sephadex G25 column chromatography to give hydroxyethylated Abps (AbpsHE) , which was identified by some physicochemical properties. By the methylation method and GCMS analysis , it is concluded that the hydroxyethyl groups are mainly substituted on 6position of the glucopyranose and fructofuranose of the sugar backbone. The product possesses antitumor activity and improves NK cell activity i