8 362-365 工艺技术 转369.20090918核桃仁种皮多糖的提取及抑菌作用的研究4012A.pdfVIP

现代食品科技 Modern Food Science and Technology 2010, Vol.26, No.4 核桃仁种皮多糖的提取及抑菌作用的研究 刘安军，王玥玮，朱振元，王妍，陈影 （天津科技大学食品工程与生物技术学院，天津300457） 摘要：本文以 0.25 mol/L 的NaOH- 乙醇溶液作提取剂，从核桃仁种皮中提取多糖，提取液经去脂、去蛋白、醇析后得粗多糖WKPP-70 。利用DEAE-Sephadex A- 25 凝胶柱和 Sephadex G-75 凝胶柱对粗多糖进行分离纯化，得纯多糖WKPP-70-1- 1。通过紫外光谱分析证明其纯度，红外光谱分析显示其具有多糖类的特征吸收峰。抑菌试验表明，WKPP-70-1-1 对大肠杆菌、沙门氏菌、枯草芽孢杆菌、金黄葡萄球菌均有一定的抑制作用。 关键词：核桃仁种皮；多糖；提取；纯化；抑菌作用 文章篇号：1673-9078(2010)4-362-365 Extraction and Antimicrobial Activities of Polysaccharide from Walnut Kernel Pellicle LIU An-jun, WANG Yue-wei, ZHU Zen-yuan, WANG Yan, CHEN Ying (College of food science and biotechnology, TianJin University of Science and Technology, TianJin 300457, China) Abstract: Crude polysaccharide, WKPP-70, was extracted from walnut kernel pellicle, using 0.25 mol/L NaOH-ethanol. After removal of lipids and proteins, WKPP-70 was further purified by chromatography on DEAE-Sephadex A-25 and Sephadex G-75 columns. The purity of the obtained polysaccharide, named as WKPP-70-1-1 was determined using UV spectrophotometric method and its structure was analyzed by infrared spectrum (IR). The antimicrobial activities of WKPP-70-1-1 against E.scherichia coli, Bacillussubtilis, Staphy lococcus aureus and SalmonellaTyphimurium were also evaluated. The results showed that WKPP-70-1-1 had antimicrobial activities against these examined microorganisms. Key words: Walnut kernel pellicle; polysaccharide; extraction; purification; antimicrobial activity 362 核桃( Juglans regia. L) 傅立叶变换红外光谱仪、BCM-1000A [1] 又名胡桃、羌桃，属胡桃科胡桃属植物 。目 型生物洁净工作台、电热恒温培养箱。 前，国内外对于核桃的研究和加工利用主要 DEAE-Sephadex A-25 、Sephadex G- 是在核桃仁方面，核桃仁含多种维生素，富 75，均为鼎