川_黔地区金钗石斛多糖的含量测定.pdfVIP

川、黔地区金钗石斛多糖的含量测定Δ 1＊ 2# 杨 艳 ，徐应淑 （1.遵义医学院附属医院药剂科，遵义市 563003；2.遵义医学院药学院，遵义市 563003） 中图分类号 R284.1；R927.2 文献标识码 A 文章编号 1001- 0408（2010）27- 2552- 03 摘 要 目的：比较川、黔金钗石斛中有效成分多糖的含量，建立金钗石斛中多糖的含量测定方法。方法：采用水提醇沉法提取金 钗石斛多糖，以葡萄糖为对照品，在紫外检测波长490nm 处用苯酚- 硫酸法测定不同产地金钗石斛中多糖的含量。结果：金钗石 斛多糖的检测浓度在1.25～8.75μg ·mL- 1范围内与吸光度呈良好线性关系（r ＝0.9994）。贵州赤水金钗石斛的平均回收率为 101.6％，RSD ＝1.9％（n ＝6），多糖含量为11.456％；四川合江金钗石斛的多糖含量为9.226％。结论：苯酚- 硫酸法操作简便、可 靠、准确、灵敏、稳定性好，适用于金钗石斛中多糖的含量测定。在川、黔地区金钗石斛多糖提取生产过程中，可优先选用贵州赤水 金钗石斛。 关键词 金钗石斛；多糖；苯酚- 硫酸法；含量；四川；贵州 Content Determination of Polysaccharide from Dendrobium nobile Cultivated in Guizhou and Sichuan YANG Yan （Dept. of Pharmacy ，The Affiliated Hospital of Zunyi Medical College ，Zunyi 563003，China ） XU Ying-shu （School of Pharmacy ，Zunyi Medical College ，Zunyi 563003，China ） ABSTRACT OBJECTIVE ：To compare the content of polysaccharide from Dendrobium nobile cultivated in Guizhou and Sich- uan ，and to establish content determination method of polysaccharide from D. nobile . METHODS ：Polysaccharide was extracted from D. nobile using water extraction alcohol precipitation. The content of polysaccharide from D. nobile cultivated in different ar- eas was determined by phenol- sulfuric acid method with glucose as standard substance at UV wavelength of 490 nm. RESULTS ： The linear range of polysaccharide from D. nobile was 1.25～8.75μg ·mL- 1（r ＝0.9994）. The average recovery of D. nobile cultivat- ed in Chishui of Guizhou province was 101.6％（RSD ＝1.9％，n ＝6），and the content of polysaccharide was 11.456％；the content of polysaccharide from D. nobile cultivated in Hejiang of Sichuan province was 9.226％. CONCLUSIONS ：Phenol- sulfuric acid meth- od is simple ，reliable ，accurate ，sensitive and stable for the content determination of polysaccharide from D. nobile. D. nobile culti- vated in Chishui of Guizhou province is the best choice for the ex