子痫前期孕妇血清与胎盘尿紧张素Ⅱ的表达及意义.pdfVIP

现代妇产科进展2007年l2月第l6卷第l2期 Prog Obstet Gynecol，Dec．2007，Vo1．16，No．12 · 论著 · 子痫前期孕妇血清与胎盘尿紧张素Ⅱ 的表达及意义 吕莉娟，乔福元 (华中科技大学同济医学院附属同济医院妇产科，武汉 430030) 【摘要】 目的：检测子痫前期孕妇外周血和新生儿脐血血清中尿紧张素Ⅱ(urotensin Ⅱ，uⅡ)水平和u lI mRNA在胎盘组织的表达水平，探讨其在子痫前期发生发展中的作 用。方法：(1)ELISA测定30例子痫前期患者及l5例正常晚期妊娠孕妇(对照组)外周 血和新生儿脐血血清UⅡ水平；(2)用RT-PCR法检测各组孕妇胎盘组织中U lI mRNA的 表达水平。结果：(1)重度子痫前期组孕妇外周血血清uⅡ水平明显高于轻度子痫前期 组和对照组，差异均有显著性(P0．05)。轻度和重度子痫前期组的脐静脉血清UⅡ浓 度均明显高于对照组(P0．05，P0．001)。(2)胎盘组织U lI mRNA表达水平在轻度和 重度子痫前期组均明显高于对照组，差异有显著性(P0．05)。结论：U lI可能参与子痫 前期全身小血管痉挛的机制并在胎盘组织缺血缺氧和动脉粥样硬化的发生起重要作用。 【关键词】 子痫前期；胎盘；血清；尿紧张素lI；动脉硬化 中图分类号：R733．33 文献标识码：A 文章编号：1004—7379(2007)12—0885—03 The expression of urotensin-lI in both seruln and placenta of patients with preeclamp- sia．La Liiuan，Q Fuyuan．Departmant of Obstetrics and Gynecology，Tongii Hospital，rongii Medical College ofHuazhong University ofScience and Technology，Wuhan 430030 【Abstract】 Objective：To study the levels of urotensin lI in maternal serum and umbili- cal cord serllm，and the mRNA expression of urotensin lI in placenta of preeclampsia patients and their relation to the pathogenesis Of preeclampsia．Me~ods：(1)ELISA was used to detect maternal and umbilical cord serum UⅡlevels in 30 patients with preeclampsia and 1 5 normal term pregnant women(control group)．(2)Expression of U lI mRNA in placenta from these groups was detected by semi-quantitative RT-PCR．Results：(1)UⅡlevels in maternal sel3．1m of severe preeclampsia group was significantly higher than that in mild preeclampsia and control group(P0．05)．UⅡlevels in umbilical cord serum of mild and severe preeclampsia groups were both significandy higher than that in control group(P0．05 and P0．001，respective- ly)．(2)Expression of U lI mRNA in placenta of mild and severe preeclampsia groups were sig- nitlcandy higher than that in control group(P0．05)．Conclusion：UⅡmay induce small maternal vessels spasmodism in